Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes produced in Escherichia coli | Streptococcus mutans |
Protein Variants | Comment | Organism |
---|---|---|
W238A | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
W238N | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
W238P | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.549 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
1.03 | - |
panose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
2.2 | - |
panose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.17 | - |
isomaltotriose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
4.22 | - |
panose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
4.96 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
5.1 | - |
isomaltopentaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
5.58 | - |
isomaltotetraose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
6.41 | - |
panose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
7.77 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
7.88 | - |
isomaltohexaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
8.91 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
9.35 | - |
isomaltose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
9.92 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
10.2 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
11.4 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
12 | - |
isomaltoheptaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
15.1 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
15.8 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
16.1 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
19 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
19.9 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
20.5 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
20.9 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
24.6 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
26.9 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
27.1 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
38.3 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
41.4 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
43.6 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
76.9 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
81.7 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
wild-type enzyme, gel filtration | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | Q2HWU5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | 377% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 4-nitrophenol + D-glucose | - |
? | |
dextran + H2O | - |
Streptococcus mutans | alpha-D-glucose | - |
? | |
isomaltoheptaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
isomaltohexaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
isomaltopentaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
isomaltose + H2O | - |
Streptococcus mutans | 2 D-glucose | - |
? | |
isomaltotetraose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
isomaltotriose + H2O | 221% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
kojibiose + H2O | 4.02% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
maltose + H2O | 0.0135% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
maltotetraose + H2O | 0.0108% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | maltotriose + D-glucose | - |
? | |
maltotriose + H2O | 0.146% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | maltose + D-glucose | - |
? | |
additional information | no activity with trehalose | Streptococcus mutans | ? | - |
? | |
nigerose + H2O | 1.99% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
panose + H2O | 417% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
sucrose + H2O | 4.29% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, wild-type enzyme, SDS-PAGE | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
dextran glucosidase | - |
Streptococcus mutans |
SMDG | - |
Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, pH 6.0, stable below | Streptococcus mutans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
0.04 | 1.97 | isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3 | 6 | isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
7.8 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
8.88 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
8.96 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
9.21 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
9.81 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
12.2 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
22.3 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
22.4 | - |
panose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
22.6 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
33.1 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
33.2 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
34.5 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
39.6 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
42.1 | - |
panose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
42.4 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
52.5 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
56.5 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
61.4 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
77.5 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
79.8 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
121 | - |
panose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
202 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
235 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
401 | - |
isomaltohexaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
419 | - |
isomaltopentaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
449 | - |
isomaltoheptaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
483 | - |
isomaltose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
499 | - |
isomaltotetraose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
538 | - |
panose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
541 | - |
isomaltotriose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
1202 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptococcus mutans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.4 | 4°C, stable for 18 h | Streptococcus mutans |