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Literature summary for 3.2.1.68 extracted from

  • Utsumi, Y.; Utsumi, C.; Sawada, T.; Fujita, N.; Nakamura, Y.
    Functional diversity of isoamylase oligomers: the ISA1 homo-oligomer is essential for amylopectin biosynthesis in rice endosperm (2011), Plant Physiol., 156, 61-77.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Star cells Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Oryza sativa
-
-

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cultivar Kinmaze
-

Purification (Commentary)

Purification (Comment) Organism
HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?

Subunits

Subunits Comment Organism
homooligomer
-
 Oryza sativa

Synonyms

Synonyms Comment Organism
ISA1
-
 Oryza sativa
ISA2
-
 Oryza sativa

General Information

General Information Comment Organism
physiological function the isoamylase 1 is essential for amylopectin biosynthesis and starch production in rice endosperm. Overexpression of the isoamylase 2 gene brings about a dramatic reduction in kernel size in the dry seed and the transformants contain less than 50% of the starch in the kernel, while the content of soluble sugars, including maltodextrins, malto-oligosaccharides, and simple sugars, increase by about 8fold when compared with the host cultivar Kinmaze Oryza sativa