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Literature summary for 3.2.1.68 extracted from
- Microbial isoamylases: An overview (2011), Am. J. Food Technol., 6, 1-18.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | saccharification potential of isoamylases is employed in food industry for preparation of high glucose syrup, maltose, maltitol, trehalose, cyclodextrin and resistant starch from starch | Myroides odoratus |
| food industry | saccharification potential of isoamylases is employed in food industry for preparation of high glucose syrup, maltose, maltitol, trehalose, cyclodextrin and resistant starch from starch | Pseudomonas amyloderamosa |
| additional information | isoamylase can potentially be used in the elucidation of fine structures of polysaccharides and related alpha-glucans and can be also be used as effective additive in dishwashing and laundry detergents | Myroides odoratus |
| additional information | isoamylase can potentially be used in the elucidation of fine structures of polysaccharides and related alpha-glucans and can be also be used as effective additive in dishwashing and laundry detergents | Pseudomonas amyloderamosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Myroides odoratus |
| expressed in Saccharomyces cerevisiae | Pseudomonas amyloderamosa |
General Stability
| General Stability | Organism |
|---|---|
| Pseudomonas isoamylase immobilized physically by raw starch adsorption results in an increase in pH and temperature stability and a retention of about 75% of activity even after 75 days | Pseudomonas amyloderamosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
x * 83000, SDS-PAGE | Myroides odoratus |
| 95000 | - |
x * 95000, SDS-PAGE | Pseudomonas amyloderamosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Myroides odoratus | - |
- |
- |
| Pseudomonas amyloderamosa | P10342 | - |
- |
| Pseudomonas amyloderamosa SB-15 | P10342 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE cellulose column chromatography, CM-cellulose column chromatography, and DEAE-Sephadex gel filtration | Myroides odoratus |
| raw starch desorption adsorption | Pseudomonas amyloderamosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-limit dextrin + H2O | - |
Myroides odoratus | linear maltooligosaccharides | - |
? |  |
| alpha-limit dextrin + H2O | - |
Pseudomonas amyloderamosa | linear maltooligosaccharides | - |
? | |
| alpha-limit dextrin + H2O | - |
Pseudomonas amyloderamosa SB-15 | linear maltooligosaccharides | - |
? | |
| amylopectin + H2O | isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin | Myroides odoratus | maltose + maltooligosaccharides | - |
? | |
| amylopectin + H2O | isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| amylopectin + H2O | isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin | Pseudomonas amyloderamosa SB-15 | maltose + maltooligosaccharides | - |
? | |
| beta-limit dextrin + H2O | - |
Myroides odoratus | linear maltooligosaccharides | - |
? | |
| beta-limit dextrin + H2O | - |
Pseudomonas amyloderamosa | linear maltooligosaccharides | - |
? | |
| beta-limit dextrin + H2O | - |
Pseudomonas amyloderamosa SB-15 | linear maltooligosaccharides | - |
? | |
| glycogen + H2O | - |
Myroides odoratus | maltose + maltooligosaccharides | - |
? | |
| glycogen + H2O | - |
Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| glycogen + H2O | - |
Pseudomonas amyloderamosa SB-15 | maltose + maltooligosaccharides | - |
? | |
| additional information | no activity towards pullulan | Myroides odoratus | ? | - |
? | |
| additional information | no activity towards pullulan | Pseudomonas amyloderamosa | ? | - |
? | |
| additional information | no activity towards pullulan | Pseudomonas amyloderamosa SB-15 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000, SDS-PAGE | Pseudomonas amyloderamosa |
| ? | x * 83000, SDS-PAGE | Myroides odoratus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen-6-glucanohydrolase | - |
Myroides odoratus |
| glycogen-6-glucanohydrolase | - |
Pseudomonas amyloderamosa |
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