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Literature summary for 3.2.1.68 extracted from
- Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice (1999), Planta, 208, 283-293.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isoamylase gene is a single copy in rice genome, located on chromsome 8 | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2Mo2O7 | 1 mM 22% inhibition, 10 mM 100% inhibition | Oryza sativa |  |
| beta-cyclodextrin | 29%, slightly inhibitory at 10 mM | Oryza sativa | |
| CuCl2 | 0.1 mM reduce activity to 3% | Oryza sativa | |
| HgCl2 | 1 mM, complete inhibition | Oryza sativa | |
| iodoacetate | 21%, slight inhibition | Oryza sativa | |
| p-chloromercuribenzoate | - |
Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | amylopectin 7.2 mg/ml-1, phytoglycogen 11.9 mg/ml-1, beta-limit dextrin 13.1 mg/ml-1, glycogen 21.8 mg/ml-1 | Oryza sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
- |
Oryza sativa |
| 340000 | - |
native enzyme, gel filtration, TSKgel G3000SWxl | Oryza sativa |
| 490000 | - |
native enzyme, gel filtration, TSKgel G4000SWxl | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
L. cv. Fujikari | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.2 | - |
rabbit liver glycogen | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
r | |
| glycogen + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
r | |
| phythoglycogen + H2O | - |
Oryza sativa | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | probably homo-tetramer and homohexamer | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rice isoamylase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | temperature treatment does not influence the enzyme activity | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Oryza sativa |
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Release 2023.1 (January 2023)
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