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Literature summary for 3.2.1.68 extracted from

  • Lee, E.Y.C.; Whelan, W.J.
    Glycogen and starch debranching enzymes (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 191-234.
No PubMed abstract available

Application

Application Comment Organism
analysis
-
Pseudomonas amyloderamosa
analysis structural determination of glycogen and starch components Cytophaga sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
-
Pseudomonas amyloderamosa
120000
-
-
Cytophaga sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Pseudomonas amyloderamosa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Cytophaga sp.
-
-
-
Pseudomonas amyloderamosa
-
gram-negative soil bacterium, strain SB-15
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O waxy maize amylopectin, potato amylopectin, amylopectin beta-dextrin Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
?
amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
?
amylopectin phi-dextrin + H2O substrate on which all 3 types of debranching enzymes act Pseudomonas amyloderamosa maltotetraose
-
?
glycogen + H2O cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
?
glycogen + H2O oyster glycogen, glycogen beta-dextrin, rabbit liver glycogen Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
?
glycogen + H2O or the beta-limit dextrins Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
?
additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Pseudomonas amyloderamosa ?
-
?
starch + H2O
-
Pseudomonas amyloderamosa ?
-
?

Synonyms

Synonyms Comment Organism
Cythophaga isoamylase
-
Cytophaga sp.
plant isoamylase
-
-
yeast isoamylase
-
-