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Literature summary for 3.2.1.67 extracted from
- Purification and characterization of exo-polygalacturonase from Zygoascus hellenicus V25 and its potential application in fruit juice clarification (2016), Food Sci. Biotechnol., 25, 1379-1385 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 116.31% activity at 5 mM | Zygoascus hellenicus |  |
| L-cysteine | 121.4% activity at 5 mM | Zygoascus hellenicus | |
| phenylmethylsulfonyl fluoride | 113.77% activity at 5 mM | Zygoascus hellenicus | |
| Tween 20 | 109.11% activity at 5 mM | Zygoascus hellenicus | |
| Tween 80 | 115.4% activity at 5 mM | Zygoascus hellenicus | |
| Urea | 110.38% activity at 5 mM | Zygoascus hellenicus | |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is applied in the juice clarification of tangerine, orange, grapefruit, and apple | Zygoascus hellenicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 84.27% residual activity at 10 mM | Zygoascus hellenicus | |
| Ba2+ | 85.24% residual activity at 10 mM | Zygoascus hellenicus | |
| Co2+ | 87.23% residual activity at 10 mM | Zygoascus hellenicus | |
| Fe2+ | 90.07% residual activity at 10 mM | Zygoascus hellenicus | |
| GSH | 83.26% residual activity at 10 mM | Zygoascus hellenicus | |
| Hg2+ | 31.41% residual activity at 10 mM | Zygoascus hellenicus | |
| Pb2+ | 87.53% residual activity at 10 mM | Zygoascus hellenicus | |
| phenylmethylsulfonyl fluoride | 81.4% residual activity at 10 mM | Zygoascus hellenicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Zygoascus hellenicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 140.11% activity at 10 mM | Zygoascus hellenicus | |
| K+ | 117.79% activity at 10 mM | Zygoascus hellenicus | |
| Mg2+ | 113.53% activity at 10 mM | Zygoascus hellenicus | |
| Na+ | 108.74% activity at 10 mM | Zygoascus hellenicus | |
| Zn2+ | 112.44% activity at 10 mM | Zygoascus hellenicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zygoascus hellenicus | - |
- |
- |
| Zygoascus hellenicus V25 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE cellulose chromatography, and Sephadex G-100 gel filtration | Zygoascus hellenicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin DE 30% + H2O | 100% activity | Zygoascus hellenicus | galacturonic acid | - |
? | |
| citrus pectin DE 30% + H2O | 100% activity | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
| citrus pectin DE 72% + H2O | 58.19% activity compared to citrus pectin DE 30% | Zygoascus hellenicus | galacturonic acid | - |
? | |
| citrus pectin DE 72% + H2O | 58.19% activity compared to citrus pectin DE 30% | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
| citrus pectin DE 85% + H2O | 7.18% activity compared to citrus pectin DE 30% | Zygoascus hellenicus | galacturonic acid | - |
? | |
| citrus pectin DE 85% + H2O | 7.18% activity compared to citrus pectin DE 30% | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
| additional information | no activity with xylan and carboxymethyl cellulose | Zygoascus hellenicus | ? | - |
? | |
| additional information | no activity with xylan and carboxymethyl cellulose | Zygoascus hellenicus V25 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75280, calculated from amino acid sequence | Zygoascus hellenicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-polygalacturonanase | - |
Zygoascus hellenicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Zygoascus hellenicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | the enzyme shows more than 50% activity between 35 and 70°C | Zygoascus hellenicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the purified enzyme is stable between 30 and 50°C, retaining more than 90% of its activity after 4 h, but the thermal stability of the enzyme obviously declines at 60-70°C | Zygoascus hellenicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Zygoascus hellenicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | the enzyme shows more than 70% activity at pH 3.0-7.0 | Zygoascus hellenicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | the enzyme retains more than 85% of its activity at pH 3.0-11.0 in 4 h | Zygoascus hellenicus |
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