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Literature summary for 3.2.1.67 extracted from

  • Yuan, P.; Meng, K.; Wang, Y.; Luo, H.; Shi, P.; Huang, H.; Bai, Y.; Yang, P.; Yao, B.
    A protease-resistant exo-polygalacturonase from Klebsiella sp. Y1 with good activity and stability over a wide pH range in the digestive tract (2012), Biores. Technol., 123, 171-176.
    View publication on PubMed
Search for more literature for 3.2.1.67

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation at 5 mM Klebsiella sp. Y1

Cloned(Commentary)

Cloned (Comment) Organism
gene pgu B, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21DE3 Klebsiella sp. Y1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 83% inhibition at 5 mM Klebsiella sp. Y1
Co2+ 90% inhibition at 5 mM Klebsiella sp. Y1
Cr3+ 90% inhibition at 5 mM Klebsiella sp. Y1
Cu2+ complete inhibition at 5 mM Klebsiella sp. Y1
EDTA 8% inhibition at 5 mM Klebsiella sp. Y1
Fe3+ 40% inhibition at 5 mM Klebsiella sp. Y1
K+ 25% inhibition at 5 mM Klebsiella sp. Y1
Li+ 33% inhibition at 5 mM Klebsiella sp. Y1
Mg2+ 36% inhibition at 5 mM Klebsiella sp. Y1
Mn2+ 64% inhibition at 5 mM Klebsiella sp. Y1
Na+ 29% inhibition at 5 mM Klebsiella sp. Y1
Ni2+ 94% inhibition at 5 mM Klebsiella sp. Y1
Pb2+ 88% inhibition at 5 mM Klebsiella sp. Y1
SDS 83% inhibition at 5 mM Klebsiella sp. Y1
Zn2+ 90% inhibition at 5 mM Klebsiella sp. Y1

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information predicted presence of a putative signal peptide at residues 1-24 Klebsiella sp. Y1
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72200
-
 x * 72200, about, sequence calculation Klebsiella sp. Y1

Organism

Organism UniProt Comment Textmining
Klebsiella sp. Y1 J9QQ32 gene pgu B
-
Klebsiella sp. Y1 CGMCC 4433 J9QQ32 gene pgu B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21DE3 by ncikel affinity chromatography Klebsiella sp. Y1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47
-
 purified recombinant His-tagged enzyme, substrate [(1->4)-alpha-D-galacturonide]n, pH 6.0, 40°C Klebsiella sp. Y1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a strict exo-type polygalacturonase and reduces the viscosity of polygalacturonic acid (1.0%, w/v) by 15.6% Klebsiella sp. Y1 ?
-
 ?
additional information the enzyme is a strict exo-type polygalacturonase and reduces the viscosity of polygalacturonic acid (1.0%, w/v) by 15.6% Klebsiella sp. Y1 CGMCC 4433 ?
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O polygalacturonnic acid Klebsiella sp. Y1 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O polygalacturonnic acid Klebsiella sp. Y1 CGMCC 4433 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

Subunits Comment Organism
? x * 72200, about, sequence calculation Klebsiella sp. Y1

Synonyms

Synonyms Comment Organism
exo-PGU B
-
 Klebsiella sp. Y1
exo-polygalacturonase
-
 Klebsiella sp. Y1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
 Klebsiella sp. Y1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 70 activity range, recombinant enzyme Klebsiella sp. Y1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 purified recombinant His-tagged enzyme, pH 6.0, 5-10 min, stable at 50°C, loss of over 80% activity at 60°C Klebsiella sp. Y1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Klebsiella sp. Y1

pH Range

pH Minimum pH Maximum Comment Organism
2 12 35% of maximal ativity within this range Klebsiella sp. Y1

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 6.8 under simulated alimentary tract conditions, the enzyme is very stable and active showing over 25% activity from pH 1.5 to pH 6.8 Klebsiella sp. Y1
2 12 purified recombinant His-tagged enzyme, 37°C, 1 h, over 30% activity remaining Klebsiella sp. Y1
3 9 purified recombinant His-tagged enzyme, 37°C, 1 h, over 65% activity remaining Klebsiella sp. Y1

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella sp. Y1 sequence calculation
-
 5.5

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase 28 family, GH28 Klebsiella sp. Y1