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Literature summary for 3.2.1.67 extracted from

  • Yadav, S.; Anand, G.; Dubey, A.; Yadav, D.
    Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre (2012), Biologia, 67, 1069-1074.
No PubMed abstract available
Search for more literature for 3.2.1.67

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Rhizopus arrhizus
Ca2+
-
 Rhizopus arrhizus
EDTA
-
 Rhizopus arrhizus
Hg2+ complete inhibition at 1 mM Rhizopus arrhizus
KMnO4 complete inhibition at 1 mM Rhizopus arrhizus
additional information poor inhibitory effects by K+ and Mg2+ Rhizopus arrhizus
NaN3
-
 Rhizopus arrhizus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 2.7 mg/ml with citrus polygalacturonic acid, pH 5.0, 50°C Rhizopus arrhizus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Rhizopus arrhizus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Rhizopus arrhizus
Mn2+ activates Rhizopus arrhizus
additional information poor activation by Na+ and potassium ferrocyanide Rhizopus arrhizus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75500
-
 x * 75500, SDS-PAGE Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-
Rhizopus arrhizus MTCC 1987
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracelluar enzyme 16.3fold by ammonium sulfate fractionation and anion exchange chromatography to homogeneity Rhizopus arrhizus

Source Tissue

Source Tissue Comment Organism Textmining
additional information submerged fermentation Rhizopus arrhizus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.5
-
 with citrus polygalacturonic acid, pH 5.0, 50°C Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-galacturonide]n + H2O citrus polygalacturonic acid Rhizopus arrhizus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O citrus polygalacturonic acid Rhizopus arrhizus MTCC 1987 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

Subunits Comment Organism
? x * 75500, SDS-PAGE Rhizopus arrhizus

Synonyms

Synonyms Comment Organism
exo-polygalacturonase
-
 Rhizopus arrhizus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhizopus arrhizus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified enzyme, 2 h, completely stable at Rhizopus arrhizus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2230
-
 [(1->4)-alpha-D-galacturonide]n pH 5.0, 50°C Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhizopus arrhizus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 12 purified enzyme, 24 h, almost completely stable at Rhizopus arrhizus

General Information

General Information Comment Organism
physiological function the enzyme plays a role in retting of Crotalaria juncea fibre Rhizopus arrhizus