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Literature summary for 3.2.1.67 extracted from
- Cloning, expression and characterization of a novel thermophilic polygalacturonase from Caldicellulosiruptor bescii DSM 6725 (2014), Int. J. Mol. Sci., 15, 5717-5729.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ACM61449, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain origami(DE3) | Caldicellulosiruptor bescii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 68% inhibition at 1 mM | Caldicellulosiruptor bescii |  |
| Ca2+ | 48% inhibition at 1 mM | Caldicellulosiruptor bescii | |
| Cd2+ | complete inhibition at 1 mM | Caldicellulosiruptor bescii | |
| Cu2+ | complete inhibition at 1 mM | Caldicellulosiruptor bescii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
| 51000 | - |
x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [(1->4)-alpha-D-galacturonide]n + H2O | Caldicellulosiruptor bescii | the enzyme releases monogalacturonic acid from its nonreducing end | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
| [(1->4)-alpha-D-galacturonide]n + H2O | Caldicellulosiruptor bescii DSM 6725 | the enzyme releases monogalacturonic acid from its nonreducing end | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor bescii | - |
gene ACM61449 | - |
| Caldicellulosiruptor bescii DSM 6725 | - |
gene ACM61449 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain origami(DE3) by heat treatment for 30 min at 70°C, and nickel affinity chromatography | Caldicellulosiruptor bescii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is specifically active towards the alpha-1,4-galacturonic acid linkages of galactopolysaccharides, no activity on Avicel, carboxymethyl cellulose, xylan, glucan, and soluble starch. A higher degree of pectin methylation corresponds to a lower activity of the enzyme | Caldicellulosiruptor bescii | ? | - |
? | |
| additional information | the enzyme is specifically active towards the alpha-1,4-galacturonic acid linkages of galactopolysaccharides, no activity on Avicel, carboxymethyl cellulose, xylan, glucan, and soluble starch. A higher degree of pectin methylation corresponds to a lower activity of the enzyme | Caldicellulosiruptor bescii DSM 6725 | ? | - |
? | |
| [(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end | Caldicellulosiruptor bescii | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
| [(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end, galacturonic acid is the only product of degradation | Caldicellulosiruptor bescii | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
| [(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end | Caldicellulosiruptor bescii DSM 6725 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
| [(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end, galacturonic acid is the only product of degradation | Caldicellulosiruptor bescii DSM 6725 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CbPelA | - |
Caldicellulosiruptor bescii |
| EPG | - |
Caldicellulosiruptor bescii |
| exo-polygalacturonase | - |
Caldicellulosiruptor bescii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 72 | - |
- |
Caldicellulosiruptor bescii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 75 | over 90% of maximal activity within this range | Caldicellulosiruptor bescii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant His-tagged enzyme, half-life is 14 h | Caldicellulosiruptor bescii |
| 80 | - |
purified recombinant His-tagged enzyme, half-life is 90 min | Caldicellulosiruptor bescii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Caldicellulosiruptor bescii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | purified recombinant His-tagged enzyme, 80% activity remaining | Caldicellulosiruptor bescii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the polygalacturonase catalyzes the hydrolysis of the homo-galacturonan skeleton | Caldicellulosiruptor bescii |
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