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Literature summary for 3.2.1.67 extracted from
- Purification and characterization of the exopolygalacturonase produced by Aspergillus giganteus in submerged cultures (2010), J. Ind. Microbiol. Biotechnol., 37, 567-573.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Aspergillus giganteus |  |
| dithiothreitol | activates | Aspergillus giganteus | |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | polygalacturonases are widely used in the food industry for juice extraction and clarification | Aspergillus giganteus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Aspergillus giganteus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Aspergillus giganteus | |
| Mg2+ | activates | Aspergillus giganteus | |
| Mn2+ | activates | Aspergillus giganteus | |
| additional information | no effect by Pb2+ | Aspergillus giganteus | |
| Na+ | activates | Aspergillus giganteus | |
| NH4+ | activates | Aspergillus giganteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69700 | - |
x * 69700, SDS-PAGE | Aspergillus giganteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | Aspergillus giganteus | - |
galacturonic acid + ? | - |
? | |
| citric pectin + H2O | Aspergillus giganteus CCT 3232 | - |
galacturonic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus giganteus | - |
- |
- |
| Aspergillus giganteus CCT 3232 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 28.1fold from submerged culture by ammonium sulfate fractionation and anion exchange chromatography | Aspergillus giganteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submerged culture | growth on citric pectin as sole carbon source | Aspergillus giganteus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 419.4 | - |
purified enzyme | Aspergillus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apple pectin + H2O | methylation degree of 75% | Aspergillus giganteus | galacturonic acid + ? | - |
? | |
| apple pectin + H2O | methylation degree of 75% | Aspergillus giganteus CCT 3232 | galacturonic acid + ? | - |
? | |
| citric pectin + H2O | - |
Aspergillus giganteus | galacturonic acid + ? | - |
? | |
| citric pectin + H2O | methylation degrees of 34-90% | Aspergillus giganteus | galacturonic acid + ? | - |
? | |
| citric pectin + H2O | - |
Aspergillus giganteus CCT 3232 | galacturonic acid + ? | - |
? | |
| citric pectin + H2O | methylation degrees of 34-90% | Aspergillus giganteus CCT 3232 | galacturonic acid + ? | - |
? | |
| additional information | purified enzyme shows its highest hydrolytic activity with low-esterified and non-esterified substrates, releasing monogalacturonic acid from substrate | Aspergillus giganteus | ? | - |
? | |
| additional information | purified enzyme shows its highest hydrolytic activity with low-esterified and non-esterified substrates, releasing monogalacturonic acid from substrate | Aspergillus giganteus CCT 3232 | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Aspergillus giganteus | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | - |
Aspergillus giganteus CCT 3232 | galacturonic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 69700, SDS-PAGE | Aspergillus giganteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exopolygalacturonase | - |
Aspergillus giganteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | - |
Aspergillus giganteus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | activity range, profile, overview | Aspergillus giganteus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 690 | - |
polygalacturonic acid | pH 6.0, 55°C | Aspergillus giganteus | |
| 770 | - |
citric pectin | pH 6.0, 55°C | Aspergillus giganteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus giganteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | activity range, profile, overview | Aspergillus giganteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable in neutral and alkaline media | Aspergillus giganteus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | polygalacturonases are pectinolytic enzymes that catalyze the hydrolysis of the plant cell-wall pectin backbone | Aspergillus giganteus |
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