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Literature summary for 3.2.1.67 extracted from
- Isolation, characterization and mode of action of exo-D-galacturonanase from carrot (1977), Collect. Czech. Chem. Commun., 42, 3214-3221.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable, even at high temperatures, relatively stable in acidic and neutral, unstable in alkaline media | Daucus carota |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | - |
Daucus carota |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.423 | - |
pectic acid | - |
Daucus carota | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectic acid + H2O | Daucus carota | - |
D-galacturonide + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
carrot | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Daucus carota | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
2 several forms of exo-D-galacturonanase | Daucus carota |
| 0.56 | - |
2 several forms of exo-D-galacturonanase | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-galacturonide)2 + H2O | - |
Daucus carota | D-galacturonic acid | - |
? | |
| (1,4-alpha-D-galacturonide)4 + H2O | - |
Daucus carota | (1,4-alpha-D-galacturonide)3 + D-galacturonic acid | - |
? | |
| (1,4-alpha-D-galacturonide)n + H2O | - |
Daucus carota | D-galacturonate + digalacturonic acid + trigalacturonic acid | - |
? | |
| oligo-D-galacturonate + H2O | - |
Daucus carota | D-galacturonic acid | - |
? | |
| p-nitrophenyl-alpha-D-galactopyranuronide + H2O | cleaves all substrates with 4-deoxy-L-threo-hexo-4-eno-pyranosyluronate unit at the nonreducing end | Daucus carota | D-galacturonic acid + 4-nitrophenol | - |
? | |
| pectic acid + H2O | degraded not fully | Daucus carota | D-galacturonic acid + ? | - |
? | |
| pectic acid + H2O | - |
Daucus carota | D-galacturonide + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | pectate, optimum temperature for enzyme action | Daucus carota |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | highly stable | Daucus carota |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | - |
pectate, native enzyme | Daucus carota |
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