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Literature summary for 3.2.1.65 extracted from

  • Mendez-Lorenzo, L.; Porras-Dominguez, J.R.; Raga-Carbajal, E.; Olvera, C.; Rodriguez-Alegria, M.E.; Carrillo-Nava, E.; Costas, M.; Lopez Munguia, A.
    Intrinsic levanase activity of Bacillus subtilis 168 levansucrase (SacB) (2015), PLoS ONE, 10, e0143394 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
 sucrose at pH 6.0 and 25°C Bacillus subtilis
63.4
-
 low molecular weight levan at pH 6.0 and 25°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
high molecular weight levan + H2O minor activity Bacillus subtilis D-fructose + ?
-
 ?
high molecular weight levan + H2O minor activity Bacillus subtilis 168 D-fructose + ?
-
 ?
low molecular weight levan + H2O minor activity Bacillus subtilis D-fructose + ?
-
 ?
low molecular weight levan + H2O minor activity Bacillus subtilis 168 D-fructose + ?
-
 ?
additional information enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose Bacillus subtilis ?
-
 ?
additional information enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose Bacillus subtilis 168 ?
-
 ?
sucrose + H2O
-
 Bacillus subtilis D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Bacillus subtilis 168 D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
levansucrase the enzyme has an exo-type levanase activity Bacillus subtilis
SacB the enzyme has an exo-type levanase activity Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.3
-
 low molecular weight levan at pH 6.0 and 25°C Bacillus subtilis
164.6
-
 sucrose at pH 6.0 and 25°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.3
-
 low molecular weight levan at pH 6.0 and 25°C Bacillus subtilis
71.2
-
 sucrose at pH 6.0 and 25°C Bacillus subtilis