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Literature summary for 3.2.1.65 extracted from
- Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs (2019), Carbohydr. Polym., 208, 285-293 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is used for enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs | Paenibacillus amylolyticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Bacillus subtilis | Paenibacillus amylolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Paenibacillus amylolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl Sepharose column chromatography and Q Sepharose chromatography | Paenibacillus amylolyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the levanase preparation exhibits an activity of 1116 U/ml towards levan | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Erwinia herbicola levan + H2O | - |
Paenibacillus amylolyticus | D-fructose + fructooligosacchrides | - |
? |  |
| levan + H2O | commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase | Aspergillus niger | ? | - |
? | |
| additional information | in order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase (EC 3.2.1.80) for levan hydrolysis achieving about 80% hydrolysis yield, method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method | Aspergillus niger | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endolevanase | - |
Aspergillus niger |
| endolevanase | - |
Paenibacillus amylolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus niger |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 32, GH32 | Aspergillus niger |
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Release 2023.1 (January 2023)
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