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Literature summary for 3.2.1.65 extracted from
- Efficient simultaneous saccharification and fermentation of inulin to 2,3-butanediol by thermophilic Bacillus licheniformis ATCC 14580 (2014), Appl. Environ. Microbiol., 80, 6458-6464.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is used for simultaneous saccharification and fermentation of inulin to 2,3-butanediol. A fed-batch simultaneous saccharification and fermentation yields 103.0 g/liter 2,3-butanediol in 30 h, with a high productivity of 3.4 g/liter/h | Bacillus licheniformis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sacC, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3) | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, recombinnat enzyme, SDS-PAGE | Bacillus licheniformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | Bacillus licheniformis | - |
? | - |
? |  |
| inulin + H2O | Bacillus licheniformis ATCC 14580 | - |
? | - |
? | |
| additional information | Bacillus licheniformis | a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C | ? | - |
? | |
| additional information | Bacillus licheniformis ATCC 14580 | a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q65GW7 | gene sacC | - |
| Bacillus licheniformis ATCC 14580 | Q65GW7 | gene sacC | - |
| no activity in Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.9 | - |
crude recombinant enzyme, pH 6.5, 50°C, substrate inulin | Bacillus licheniformis |
| 987 | - |
purified recombinant enzyme, pH 6.5, 50°C, substrate inulin | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| inulin + H2O | - |
Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| additional information | a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C | Bacillus licheniformis | ? | - |
? | |
| additional information | a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, recombinnat enzyme, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | activity range, profile overview | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant enzyme, pH 6.5, 1 h, stable up to | Bacillus licheniformis |
| 55 | - |
purified recombinant enzyme, pH 6.5, 1 h, 89% activity remaining | Bacillus licheniformis |
| 65 | - |
purified recombinant enzyme, pH 6.5, 1 h, inactivation | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | higher activity, recombinant enzyme | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | purified recombinant enzyme, 50°C, 1 h, stable at | Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 32, GH32 | Bacillus licheniformis |
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