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Literature summary for 3.2.1.65 extracted from

  • Menendez, C.; Banguela, A.; Caballero-Mellado, J.; Hernandez, L.
    Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus (2009), Appl. Environ. Microbiol., 75, 1782-1785.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.65

Cloned(Commentary)

Cloned (Comment) Organism
gene lsdB, organized in one operon with gene lsdA, encoding levansucrase, organization of the lsdAB operon and relevant features of the intergenic sequence, expression analysis, overview Gluconacetobacter diazotrophicus

Protein Variants

Protein Variants Comment Organism
additional information type II secretion mutants AD6, AD8, and AD9, unable to secrete LsdA due to nptII insertions in the type II secretion, the mutants fail to transfer LsdB across the outer membrane, impeding levan hydrolysis Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Gluconacetobacter diazotrophicus
-
-
additional information mature LsdB is unlikely to attach cell membranes Gluconacetobacter diazotrophicus
-
-

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
 gene lsdB
-
Gluconacetobacter diazotrophicus SRT4
-
 gene lsdB
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme expression analysis under different growth conditions, overview Gluconacetobacter diazotrophicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
 intracellular wild-type enzyme Gluconacetobacter diazotrophicus
0.33
-
 extracellular mutant AD6 enzyme Gluconacetobacter diazotrophicus
0.52
-
 extracellular mutant AD8 enzyme Gluconacetobacter diazotrophicus
0.69
-
 intracellular mutant AD9 enzyme Gluconacetobacter diazotrophicus
0.71
-
 extracellular mutant AD9 enzyme Gluconacetobacter diazotrophicus
0.73
-
 intracellular mutant AD8 enzyme Gluconacetobacter diazotrophicus
0.75
-
 intracellular mutant AD6 enzyme Gluconacetobacter diazotrophicus
14.24
-
 extracellular wild-type enzyme Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan + H2O substrate from Erwinia herbicola Gluconacetobacter diazotrophicus ?
-
 ?
levan + H2O substrate from Erwinia herbicola Gluconacetobacter diazotrophicus SRT4 ?
-
 ?

Synonyms

Synonyms Comment Organism
exolevanase
-
 Gluconacetobacter diazotrophicus
LsdB
-
 Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Gluconacetobacter diazotrophicus

Expression

Organism Comment Expression
Gluconacetobacter diazotrophicus lsdB transcription is repressed by D-glucose down
Gluconacetobacter diazotrophicus lsdB transcription is induced during growth with low fructose concentrations of 0.44 to 33 mM up