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Literature summary for 3.2.1.65 extracted from

  • Miasnikov, A.N.
    Characterization of a novel endo-levanase and its gene from Bacillus sp. L7 (1997), FEMS Microbiol. Lett., 154, 23-28.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in B. subtilis Bacillus sp. L7

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Bacillus sp. L7
Ca2+
-
Bacillus sp. L7
Cu2+
-
Bacillus sp. L7
Fe3+
-
Bacillus sp. L7
Hg2+
-
Bacillus sp. L7
Mg2+
-
Bacillus sp. L7
Mn2+
-
Bacillus sp. L7
Na+
-
Bacillus sp. L7
sucrose weak inhibitory effect at high concentrations Bacillus sp. L7
Zn2+
-
Bacillus sp. L7

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. L7
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
SDS-PAGE and DNA sequence analysis Bacillus sp. L7

Organism

Organism UniProt Comment Textmining
Bacillus sp. L7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. L7

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
128
-
U/mg Bacillus sp. L7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan + H2O
-
Bacillus sp. L7 fructose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. L7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
no activity loss at pH 6.0 and 60°C for 20 min Bacillus sp. L7