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Literature summary for 3.2.1.65 extracted from
- Purification and charakterization of a levanase from Streptomyces sp. 366L (1998), J. Biotechnol., 61, 33-41.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | plant differentiation, growth promoting activities for some enteric bacteria | Streptomyces sp. 366L |
| medicine | enhancing effect on the immune system and other physiological functions | Streptomyces sp. 366L |
| nutrition | energy source | Streptomyces sp. 366L |
| nutrition | production of noncaloric sweeteners | Streptomyces sp. 366L |
| synthesis | protection from aging | Streptomyces sp. 366L |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Streptomyces sp. 366L |  |
| Co2+ | - |
Streptomyces sp. 366L | |
| iodoacetate | - |
Streptomyces sp. 366L | |
| Mn2+ | - |
Streptomyces sp. 366L | |
| p-chloromercuribenzoate | - |
Streptomyces sp. 366L | |
| SDS | - |
Streptomyces sp. 366L | |
| Zn2+ | - |
Streptomyces sp. 366L | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
SDS-PAGE | Streptomyces sp. 366L |
| 80000 | - |
gel filtration | Streptomyces sp. 366L |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. 366L | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces sp. 366L |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4309 | - |
U/mg | Streptomyces sp. 366L |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| levan + H2O | - |
Streptomyces sp. 366L | levanheptaose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE | Streptomyces sp. 366L |
| monomer | gel filtration | Streptomyces sp. 366L |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
rapidly inactivated above 45°C | Streptomyces sp. 366L |
| 40 | - |
pH 7.0 | Streptomyces sp. 366L |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
rapidly decreased activity below and above pH 7.0 | Streptomyces sp. 366L |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 20°C | Streptomyces sp. 366L |
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