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Literature summary for 3.2.1.65 extracted from

  • Igarashi, T.; Takahashi, M.; Yamamoto, A.; Etoh, Y.; Takamori, K.
    Purification and characterization of levanase from Actinomyces viscosus ATCC19246 (1987), Infect. Immun., 55, 3001-3005.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.65

Application

Application Comment Organism
medicine pathogenicity of periodontal disease Actinomyces viscosus
medicine mitogenic for human B lymphocytes and activates the alternative complement pathway Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Actinomyces viscosus
EDTA
-
 Actinomyces viscosus
Fe3+
-
 Actinomyces viscosus
Hg2+
-
 Bacillus subtilis
Mn2+
-
 Actinomyces viscosus
Zn2+
-
 Actinomyces viscosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus subtilis
-
-
extracellular
-
 Actinomyces viscosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 SDS-PAGE Actinomyces viscosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
levans + H2O Bacillus subtilis
-
 oligofructosides
-
 ?
levans + H2O Actinomyces viscosus
-
 oligofructosides
-
 ?

Organism

Organism UniProt Comment Textmining
Actinomyces viscosus
-
-
-
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinomyces viscosus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
851
-
-
 Actinomyces viscosus

Storage Stability

Storage Stability Organism
4°C, phosphate buffer, pH 6.0 Actinomyces viscosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Bacillus subtilis ?
-
 ?
inulin + H2O
-
 Actinomyces viscosus ?
-
 ?
levan + H2O
-
 Bacillus subtilis oligofructosides
-
 ?
levan + H2O
-
 Actinomyces viscosus oligofructosides
-
 ?
levans + H2O
-
 Bacillus subtilis oligofructosides
-
 ?
levans + H2O
-
 Actinomyces viscosus oligofructosides
-
 ?
sucrose + H2O
-
 Bacillus subtilis D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Actinomyces viscosus D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Actinomyces viscosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 not active above 55°C Actinomyces viscosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 not active below Actinomyces viscosus
6
-
-
 Actinomyces viscosus