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Literature summary for 3.2.1.64 extracted from
- Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2 (2000), Appl. Environ. Microbiol., 66, 252-256.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces exfoliatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
1 * 54000, SDS-PAGE | Streptomyces exfoliatus |
| 60000 | - |
gel filtration | Streptomyces exfoliatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 levan + 7 H2O | Streptomyces exfoliatus | levan i.e. 2,6-beta-D-fructan | 10 levanbiose | 6-beta-fructosylfructose | ? |  |
| 3 levan + 7 H2O | Streptomyces exfoliatus F3-2 | levan i.e. 2,6-beta-D-fructan | 10 levanbiose | 6-beta-fructosylfructose | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces exfoliatus | - |
- |
- |
| Streptomyces exfoliatus F3-2 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Streptomyces exfoliatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 105 | - |
- |
Streptomyces exfoliatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-fructosylnystose + H2O | at 81.4% the rate of levan | Streptomyces exfoliatus | beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose + nystose | - |
? | |
| 1-kestose + H2O | at 33.3% the rate of levan | Streptomyces exfoliatus | sucrose + beta-D-fructose + alpha-D-glucose | - |
? | |
| 1-kestose + H2O | at 33.3% the rate of levan | Streptomyces exfoliatus F3-2 | sucrose + beta-D-fructose + alpha-D-glucose | - |
? | |
| 3 levan + 7 H2O | levan i.e. 2,6-beta-D-fructan | Streptomyces exfoliatus | 10 levanbiose | 6-beta-fructosylfructose | ? | |
| 3 levan + 7 H2O | smallest substrate to be hydrolyzed is levantriose | Streptomyces exfoliatus | 10 levanbiose | 6-beta-fructosylfructose | ? | |
| 3 levan + 7 H2O | levan i.e. 2,6-beta-D-fructan | Streptomyces exfoliatus F3-2 | 10 levanbiose | 6-beta-fructosylfructose | ? | |
| 3 levan + 7 H2O | smallest substrate to be hydrolyzed is levantriose | Streptomyces exfoliatus F3-2 | 10 levanbiose | 6-beta-fructosylfructose | ? | |
| nystose + H2O | at 73.4% the rate of levan | Streptomyces exfoliatus | beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose | - |
? | |
| nystose + H2O | at 73.4% the rate of levan | Streptomyces exfoliatus F3-2 | beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose | - |
? | |
| sucrose + H2O | at 6.5% the rate of levan | Streptomyces exfoliatus | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | at 6.5% the rate of levan | Streptomyces exfoliatus F3-2 | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, SDS-PAGE | Streptomyces exfoliatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Streptomyces exfoliatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Streptomyces exfoliatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | - |
Streptomyces exfoliatus |
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