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Literature summary for 3.2.1.63 extracted from
- An F-type lectin domain directs the activity of Streptosporangium roseum alpha-L-fucosidase (2018), Glycobiology, 28, 860-875 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptosporangium roseum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D244A | the enzyme has a mutation in the active site of the alpha-L-fucosidase domain | Streptosporangium roseum |
| D244A/H623A/R651A/R658A | the enzyme has a mutation in the active site of the alpha-L-fucosidase domain | Streptosporangium roseum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-fucosyllactose | - |
Streptosporangium roseum |  |
| alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc | i.e. blood group antigen B-tetraose type 2 | Streptosporangium roseum | |
| alpha-D-GalNAc-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc | i.e. blood group antigen A-tetraose type 2 | Streptosporangium roseum | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc | i.e. Lewisb tetraose | Streptosporangium roseum | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-D-Gal | i.e. blood group antigen H-tetraose type 2 | Streptosporangium roseum | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc | i.e. blood group antigen H-triose type 2 | Streptosporangium roseum | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc | i.e. Lewisy tetraose | Streptosporangium roseum | |
| beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GalNAc-(1->3)-D-Gal | i.e. Lewis a tetraose | Streptosporangium roseum | |
| beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-Glc | i.e. Lewisa hexaose | Streptosporangium roseum | |
| beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-(1->3)-D-Gal | i.e. Lewisx tetraose | Streptosporangium roseum | |
| Co2+ | 0.8fold decrease of activity at 10 mM | Streptosporangium roseum | |
| Cu2+ | 1.0fold decrease of activity at 10 mM | Streptosporangium roseum | |
| deoxyfuconojirimycin | - |
Streptosporangium roseum | |
| L-fucose | - |
Streptosporangium roseum | |
| additional information | no substantial effect on alpha-L-fucosidase activity upon the addition of 10 mM EDTA or 300 mM NaCl | Streptosporangium roseum | |
| mucin | - |
Streptosporangium roseum | |
| N-acetyl-propargyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc | i.e. blood group antigen H-pentaose type-2 beta-N-acetyl propargyl | Streptosporangium roseum | |
| Ni2+ | 1.0fold decrease of activity at 10 mM | Streptosporangium roseum | |
| Zn2+ | 1.2fold decrease of activity at 10 mM | Streptosporangium roseum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01059 | - |
4-methylumbelliferyl alpha-L-fucopyranoside | at 37°C, pH not specified in the publication | Streptosporangium roseum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 93.4fold increase of activity at 10 mM Ca2+ | Streptosporangium roseum | |
| Mn2+ | 6.7fold increase of activity at 10 mM Mn2+ | Streptosporangium roseum | |
| additional information | no substantial effect on alpha-L-fucosidase activity upon the addition of 10 mM Mg2+ | Streptosporangium roseum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptosporangium roseum | D2B1P1 | - |
- |
| Streptosporangium roseum DSM 43021 | D2B1P1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-fucosyllactose + H2O | - |
Streptosporangium roseum | L-fucose + lactose | - |
? | |
| 2'-fucosyllactose + H2O | - |
Streptosporangium roseum DSM 43021 | L-fucose + lactose | - |
? | |
| 4-methylumbelliferyl alpha-L-fucopyranoside + H2O | - |
Streptosporangium roseum | 4-methylumbelliferone + L-fucopyranose | - |
? | |
| 4-methylumbelliferyl alpha-L-fucopyranoside + H2O | - |
Streptosporangium roseum DSM 43021 | 4-methylumbelliferone + L-fucopyranose | - |
? | |
| alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc + H2O | i.e. blood group antigen B-tetraose type 2, weak substrate | Streptosporangium roseum | L-fucose + ? | - |
? | |
| alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc + H2O | i.e. blood group antigen B-tetraose type 2, weak substrate | Streptosporangium roseum DSM 43021 | L-fucose + ? | - |
? | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O | i.e. blood group antigen H pentaose type-1, weak substrate | Streptosporangium roseum | L-fucose + ? | - |
? | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-D-Gal + H2O | i.e. blood group antigen H-tetraose type 2 | Streptosporangium roseum | L-fucose + ? | - |
? | |
| alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc + H2O | i.e. blood group antigen H triose type-2 | Streptosporangium roseum | L-fucose + ? | - |
? | |
| beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GalNAc-(1->3)-D-Gal | i.e. Lewis a tetraose | Streptosporangium roseum | L-fucose + ? | - |
? | |
| biotin-PAA-alpha-L-fucose + H2O | - |
Streptosporangium roseum | ? | - |
? | |
| additional information | not active with biotin-PAA-alpha-D-galactose | Streptosporangium roseum | ? | - |
? | |
| additional information | the alpha-L-fucosidase domain of the protein accepts both alpha1-2 and alpha1-4 linked fucosylated glycans as substrates and has high affinity for a Fuc-alpha-1,2-Gal motif | Streptosporangium roseum | ? | - |
? | |
| additional information | not active with biotin-PAA-alpha-D-galactose | Streptosporangium roseum DSM 43021 | ? | - |
? | |
| additional information | the alpha-L-fucosidase domain of the protein accepts both alpha1-2 and alpha1-4 linked fucosylated glycans as substrates and has high affinity for a Fuc-alpha-1,2-Gal motif | Streptosporangium roseum DSM 43021 | ? | - |
? | |
| N-acetyl-propargyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O | i.e. blood group antigen H-pentaose type-2 beta-N-acetyl propargyl | Streptosporangium roseum | L-fucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, SDS-PAGE | Streptosporangium roseum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-fucosidase | - |
Streptosporangium roseum |
| FucNaFLD | - |
Streptosporangium roseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptosporangium roseum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | more than 50% activity between 20 and 45°C | Streptosporangium roseum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 7.5 | the enzyme is optimally active at pH 5.6 to 7.5. Some alpha-L-fucosidase activity is retained at pH 9.2, but no significant activity is found at pH 2.6 and 4.5 | Streptosporangium roseum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00044 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | deoxyfuconojirimycin | |
| 0.007 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc | |
| 0.011 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc | |
| 0.019 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | N-acetyl-propargyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc | |
| 0.026 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc | |
| 0.046 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-D-Gal | |
| 0.0477 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | L-fucose | |
| 0.076 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-D-GalNAc-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc | |
| 0.139 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc | |
| 0.198 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-Glc | |
| 0.255 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-(1->3)-D-Gal | |
| 0.293 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GalNAc-(1->3)-D-Gal | |
| 0.485 | - |
at 37°C, pH not specified in the publication | Streptosporangium roseum | 3'-fucosyllactose | |
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