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Literature summary for 3.2.1.63 extracted from
- Purification and properties of alpha-L-fucosidase from Bacillus fulminans (1973), J. Biochem., 74, 1141-1149.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | - |
Bacillus fulminans |  |
| EDTA | - |
Bacillus fulminans | |
| Hg2+ | - |
Bacillus fulminans | |
| Mn2+ | - |
Bacillus fulminans | |
| SDS | - |
Bacillus fulminans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
glycoprotein | hog, submaxillary | Bacillus fulminans | |
| 0.33 | - |
porcine submaxillary mucin | - |
Bacillus fulminans | |
| 1.6 | - |
glycopeptide desialyzed porcine submaxillary mucin | - |
Bacillus fulminans | |
| 2 | - |
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc | - |
Bacillus fulminans | |
| 2.5 | - |
2'-fucosyllactose | - |
Bacillus fulminans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | 80000 | gel filtration | Bacillus fulminans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus fulminans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus fulminans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
- |
Bacillus fulminans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-fucosyllactose + H2O | - |
Bacillus fulminans | fucose + lactose | - |
? | |
| alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc + H2O | - |
Bacillus fulminans | alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc | - |
? | |
| glycopeptide desialyzed porcine submaxillary mucin + H2O | - |
Bacillus fulminans | ? | - |
? | |
| glycoprotein + H2O | - |
Bacillus fulminans | ? | - |
? | |
| lacto-N-fucopentaose I | - |
Bacillus fulminans | ? | - |
? | |
| mucin + H2O | porcine submaxillary and glycopeptide of desialyzed mucin | Bacillus fulminans | L-fucose + ? | - |
? | |
| O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O | i.e. 2-O-alpha-L-fucosyllactose | Bacillus fulminans | L-fucose + lactose | - |
? | |
| porcine submaxillary mucin + H2O | - |
Bacillus fulminans | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, no loss of activity | Bacillus fulminans |
| 60 | - |
5 min, 7% loss of activity | Bacillus fulminans |
| 70 | - |
5 min, 77% loss of activity | Bacillus fulminans |
| 80 | - |
5 min, complete loss of activity | Bacillus fulminans |
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