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Literature summary for 3.2.1.61 extracted from

  • Tung, K.K.; Rosenthal, A.; Nordin, J.H.
    Enzymes that hydrolyze fungal cell wall polysaccharides. II. Purification and properties of mycodextranase, an endo -D-(1-4) glucanase from Penicillium melinii (1971), J. Biol. Chem., 246, 6722-6736.
    View publication on PubMed
Search for more literature for 3.2.1.61

Inhibitors

Inhibitors Comment Organism Structure
Ag+ fast enzyme Penicillium melinii
Hg+ fast enzyme Penicillium melinii
maltose
-
 Penicillium melinii
maltotriose
-
 Penicillium melinii
N-bromosuccinimide fast enzyme Penicillium melinii
N-Chlorosuccinimide fast enzyme Penicillium melinii
nigeran tetrasaccharide
-
 Penicillium melinii
nigeran trisaccharide
-
 Penicillium melinii
nigerose
-
 Penicillium melinii
Urea
-
 Penicillium melinii

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 sedimentation equilibrium fast and slow enzyme Penicillium melinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nigeran + H2O Penicillium melinii
-
 nigerose + 4-alpha-D-nigerosylglucose
-
 ?
nigeran + H2O Penicillium melinii QM 1931
-
 nigerose + 4-alpha-D-nigerosylglucose
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium melinii
-
 QM 1931
-
Penicillium melinii QM 1931
-
 QM 1931
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein fast form: 16% carbohydrate, slow form: 15% carbohydrate, only mannose and glucose Penicillium melinii

Purification (Commentary)

Purification (Comment) Organism
fast and slow enzyme, different electrophoretic mobilities Penicillium melinii

Reaction

Reaction Comment Organism Reaction ID
nigerohexaose + H2O = nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose endo-multichain mechanism Penicillium melinii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
-
 Penicillium melinii

Storage Stability

Storage Stability Organism
4°C, 2 months, no loss of activity, fast and slow enzyme Penicillium melinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nigeran + H2O both, fast and slow enzyme Penicillium melinii nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose no production of glucose ?
nigeran + H2O both, fast and slow enzyme Penicillium melinii QM 1931 nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose no production of glucose ?
nigeran + H2O
-
 Penicillium melinii nigerose + 4-alpha-D-nigerosylglucose
-
 ?
nigeran + H2O
-
 Penicillium melinii QM 1931 nigerose + 4-alpha-D-nigerosylglucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, guanidine hydrochloride treatment Penicillium melinii
More fast and slow enzyme have similar but not identical amino acid compositions Penicillium melinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 fast and slow enzyme Penicillium melinii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 80 23°C: fast enzyme 7%, slow enzyme 16% of maximal activity, 80°C: fast enzyme 50%, slow enzyme 30% of maximal activity Penicillium melinii

pH Range

pH Minimum pH Maximum Comment Organism
2 9 fast and slow enzyme pH 2 15%, pH 9 75% of maximal activity Penicillium melinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 stable between Penicillium melinii