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Literature summary for 3.2.1.60 extracted from

  • Kobayashi, S.; Takaki, Y.; Kobata, K.; Takami, H.; Inoue, A.
    Characterization of maltotetraohydrolase produced Pseudomonas sp. MS300 isolated from the deepest site of the Mariana Trench (1998), Extremophiles, 2, 401-407.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM CuCl2, amylase A, 98% inhibition, amylase B, complete inhibition Pseudomonas sp.
Fe3+ 1 mM FeCl3, amylase A, 73% inhibition, amylase B, 87% inhibition Pseudomonas sp.
Hg2+ 1 mM HgCl2, amylase A, 99% inhibition, amylase B, 97% inhibition Pseudomonas sp.
Zn2+ 1 mM ZnCl2, amylase A 99% inhibition, amylase B, 97% inhibition Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. more amylase production under high hydrostatic pressure than under atmospheric pressure ?
-
?
additional information Pseudomonas sp. MS300 more amylase production under high hydrostatic pressure than under atmospheric pressure ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
MS300
-
Pseudomonas sp. MS300
-
MS300
-

Purification (Commentary)

Purification (Comment) Organism
amylase A and B Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1544
-
amylase A Pseudomonas sp.
1775
-
amylase B Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information more amylase production under high hydrostatic pressure than under atmospheric pressure Pseudomonas sp. ?
-
?
additional information more amylase production under high hydrostatic pressure than under atmospheric pressure Pseudomonas sp. MS300 ?
-
?
starch + H2O amylase A and B potato starch Pseudomonas sp. maltotetraose
-
?
starch + H2O amylase A and B potato starch Pseudomonas sp. MS300 maltotetraose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
amylase A and B Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
amylase A and B, pH 7, full activity below Pseudomonas sp.
60
-
10 min, amylase A and B, inactivation Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
amylase A, 40°C Pseudomonas sp.
7.8
-
amylase B, 40°C Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 amylase B, 40°C, 30 min, stable between Pseudomonas sp.
7 8 amylase A, 40°C, 30 min, stable between Pseudomonas sp.