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Literature summary for 3.2.1.6 extracted from
- Overexpression and characterization of a novel endo-beta-1,3(4)-glucanase from thermophilic fungus Humicola insolens Y1 (2017), Protein Expr. Purif., 138, 63-68 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Humicola insolens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 93% residual activity | Humicola insolens |  |
| Ag+ | 1 mM, no residual activity | Humicola insolens | |
| Ca2+ | 1 mM, 83% residual activity | Humicola insolens | |
| EDTA | 1 mM, 91% residual activity | Humicola insolens | |
| Mn2+ | 1 mM, 66% residual activity | Humicola insolens | |
| SDS | 1 mM, 75% residual activity | Humicola insolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Barley beta-glucan | Km value 0.91 mg/ml, pH 5.5, 55°C | Humicola insolens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humicola insolens | A0A0U2RV57 | - |
- |
| Humicola insolens Y1 | A0A0U2RV57 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 693 | - |
substrate barley beta-glucan, pH 5.5, 556°C | Humicola insolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Humicola insolens | ? | - |
? | |
| barley beta-glucan + H2O | - |
Humicola insolens Y1 | ? | - |
? | |
| laminarin + H2O | - |
Humicola insolens | ? | - |
? | |
| laminarin + H2O | - |
Humicola insolens Y1 | ? | - |
? | |
| lichenan + H2O | - |
Humicola insolens | ? | - |
? | |
| lichenan + H2O | - |
Humicola insolens Y1 | ? | - |
? | |
| additional information | no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper | Humicola insolens | ? | - |
? | |
| additional information | no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper | Humicola insolens Y1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32400, calculated | Humicola insolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Humicola insolens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Humicola insolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Humicola insolens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Humicola insolens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Humicola insolens | calculated | - |
6 |
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