Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzylbromide | 5 mM, 47% inhibition | Laceyella putida | |
Hg2+ | 1 mM, 39% inhibition | Laceyella putida | |
iodoacetic acid | 5 mM, 34% inhibition | Laceyella putida | |
Pd2+ | 1 mM, 23% inhibition | Laceyella putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.21 | - |
laminaritetraose | pH 4.0, 50°C | Laceyella putida | |
2.39 | - |
Laminaripentaose | pH 4.0, 50°C | Laceyella putida | |
2.73 | - |
Laminarihexaose | pH 4.0, 50°C | Laceyella putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Laceyella putida | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
- |
Laceyella putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laceyella putida | A0A0P0UPC6 | - |
- |
Laceyella putida JAM FM3001 | A0A0P0UPC6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Laceyella putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
curdlan + H2O | - |
Laceyella putida | laminaribiose + laminaritriose + ? | main products | ? | |
laminarihexaose + H2O | - |
Laceyella putida | D-glucose + laminaribiose + laminaritriose | - |
? | |
laminarin + H2O | 48% of the activity with curdlan | Laceyella putida | ? | - |
? | |
laminarin + H2O | 48% of the activity with curdlan | Laceyella putida JAM FM3001 | ? | - |
? | |
laminaripentaose + H2O | - |
Laceyella putida | D-glucose + laminaribiose + laminaritriose | - |
? | |
laminaritetraose + H2O | - |
Laceyella putida | D-glucose + laminaribiose + laminaritriose | - |
? | |
laminaritetraose + H2O | - |
Laceyella putida JAM FM3001 | D-glucose + laminaribiose + laminaritriose | - |
? | |
laminaritriose + H2O | - |
Laceyella putida | D-glucose + laminaribiose | - |
? | |
laminaritriose + H2O | - |
Laceyella putida JAM FM3001 | D-glucose + laminaribiose | - |
? | |
additional information | no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose | Laceyella putida | ? | - |
? | |
additional information | no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose | Laceyella putida JAM FM3001 | ? | - |
? | |
pachyman + H2O | 223% of the activity with curdlan | Laceyella putida | ? | - |
? | |
pachyman + H2O | 223% of the activity with curdlan | Laceyella putida JAM FM3001 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE, x * 39836, calculated | Laceyella putida |
Synonyms | Comment | Organism |
---|---|---|
GluA | - |
Laceyella putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Laceyella putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme shows relatively low thermostability, which is stabilized by adding laminarin, xylan, colloidal chitin, pectin, and its related polysaccharides | Laceyella putida |
30 | 75 | 30 min, pH 7.5, 20 mM Tris-HCl, 35-45% residual activtiy | Laceyella putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
188 | - |
laminaritetraose | pH 4.0, 50°C | Laceyella putida | |
355 | - |
Laminaripentaose | pH 4.0, 50°C | Laceyella putida | |
582 | - |
Laminarihexaose | pH 4.0, 50°C | Laceyella putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Laceyella putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.4 | 8.7 | 40°C, 30 min, about 50% residual activity | Laceyella putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Laceyella putida | calculated | - |
5 |