Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 119% of initial activity | Alicyclobacillus sp. |
Application | Comment | Organism |
---|---|---|
food industry | application in malting and brewing industry. Under simulated mashing conditions, addition of Agl9A at 20 U/ml or a commercial xylanase at 200 U/ml reduce the filtration rate by 26.71% and 20.21%, respectively, and viscosity by 6.12% and 4.78%, respectively. Combined use of Agl9A at 10 U/ml and the xylanase at 100 U/ml even more effectively reduces the filtration rate by 31.73% and viscosity by 8.79% | Alicyclobacillus sp. |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Alicyclobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 2 mM, complete loss of activity | Alicyclobacillus sp. | |
Cr3+ | 2 mM, 78% of initial activity | Alicyclobacillus sp. | |
Cu2+ | 2 mM, 23% of initial activity | Alicyclobacillus sp. | |
EDTA | 2 mM, 64% of initial activity | Alicyclobacillus sp. | |
Hg2+ | 2 mM, complete loss of activity | Alicyclobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, 113% of initial activity | Alicyclobacillus sp. | |
Mn2+ | 2 mM, 123% of initial activity | Alicyclobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE, x * 60700, calculated | Alicyclobacillus sp. |
60700 | - |
x * 60000, SDS-PAGE, x * 60700, calculated | Alicyclobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus sp. | D7PC10 | - |
- |
Storage Stability | Organism |
---|---|
4°C, 0.1 M McIlvaine buffer, pH 5.8, retains almost all initial activity after 1 week of storage | Alicyclobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | source of substrate: barley | Alicyclobacillus sp. | ? | - |
? | |
carboxymethyl-cellulose + H2O | 15% of the activity with beta-D-glucan | Alicyclobacillus sp. | ? | - |
? | |
laminarin + H2O | 6.2% of the activity with beta-D-glucan | Alicyclobacillus sp. | ? | - |
? | |
lichenan + H2O | 109% of the activity with beta-D-glucan | Alicyclobacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, x * 60700, calculated | Alicyclobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
Agl9A | - |
Alicyclobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Alicyclobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
rapid loss of activity above | Alicyclobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, more than 70% residual activity | Alicyclobacillus sp. |
70 | - |
30 min, 20% residual activity | Alicyclobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Alicyclobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 65% of maximum activity within | Alicyclobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.8 | 11.2 | 1 h, more than 60% residual activity | Alicyclobacillus sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is highly resistant to trypsin, alpha-chymotrypsin, collagenase and Neutrase 0.8L | Alicyclobacillus sp. |