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Literature summary for 3.2.1.59 extracted from

  • Ebisu, S.; Kato, K.; Kotani, S.; Misaki, A.
    Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans (1975), J. Bacteriol., 124, 1489-1501.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.59

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Flavobacterium sp.
Hg2+
-
 Flavobacterium sp.
Pb2+
-
 Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.831
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucan + H2O insoluble, sticky glucan of Streptococcus mutans Flavobacterium sp. isomaltose + nigerose + nigerotriose + oligosaccharides
-
 ?
additional information
-
 Flavobacterium sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 complete loss of activity after 30 min Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: 40% of optimal activity, pH 8.5: 70% of optimal activity Flavobacterium sp.