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Literature summary for 3.2.1.58 extracted from

  • Papageorgiou, A.C.; Chen, J.; Li, D.
    Crystal structure and biological implications of a glycoside hydrolase family 55 beta-1,3-glucanase from Chaetomium thermophilum (2017), Biochim. Biophys. Acta, 1865, 1030-1038 .
    View publication on PubMed
Search for more literature for 3.2.1.58

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Thermochaetoides thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.8 M of Na/K phosphate, pH 6.8 Thermochaetoides thermophila

Protein Variants

Protein Variants Comment Organism
D595A the mutant has fully lost enzymatic activity on laminarin Thermochaetoides thermophila
E631A the mutant has fully lost enzymatic activity on laminarin Thermochaetoides thermophila
E654A the mutant has fully lost enzymatic activity on laminarin Thermochaetoides thermophila
H596L the mutant shows 60-80% enzymatic activity on laminarin compared with the wild type enzyme Thermochaetoides thermophila
Q159L the mutant shows 60-80% enzymatic activity on laminarin compared with the wild type enzyme Thermochaetoides thermophila
Q190L the mutant has fully lost enzymatic activity on laminarin Thermochaetoides thermophila
Q241L the mutant almost loses enzymatic activity on laminarin Thermochaetoides thermophila
S220A the mutant shows 60-80% enzymatic activity on laminarin compared with the wild type enzyme Thermochaetoides thermophila
W590G the mutant shows 60-80% enzymatic activity on laminarin compared with the wild type enzyme Thermochaetoides thermophila
W592G the mutant shows 60-80% enzymatic activity on laminarin compared with the wild type enzyme Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila G0S3N2
-
-
Thermochaetoides thermophila DSM 1495 G0S3N2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarihexaose + H2O
-
 Thermochaetoides thermophila D-glucose + ?
-
 ?
laminarihexaose + H2O
-
 Thermochaetoides thermophila DSM 1495 D-glucose + ?
-
 ?
laminarin + H2O
-
 Thermochaetoides thermophila D-glucose + ?
-
 ?
laminarin + H2O
-
 Thermochaetoides thermophila DSM 1495 D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
exo-beta-1,3-glucanase
-
 Thermochaetoides thermophila
Lam55
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme is thermostable at 50°C but retains about 40% of its activity after incubation at 60°C for10 min (half-life of the enzyme at 60°C is about 8 min). Most of the enzyme activity is lost at above 70°C after 10 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermochaetoides thermophila