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Literature summary for 3.2.1.58 extracted from

  • Konno, N.; Nakade, K.; Nishitani, Y.; Mizuno, M.; Sakamoto, Y.
    Lentinan degradation in the Lentinula edodes fruiting body during postharvest preservation is reduced by downregulation of the exo-beta-1,3-glucanase EXG2 (2014), J. Agric. Food Chem., 62, 8153-8157.
    View publication on PubMed
Search for more literature for 3.2.1.58

Cloned(Commentary)

Cloned (Comment) Organism
gene exg2, recombinant overexpression in Lentinula edodes strain SR-1 Lentinula edodes

Protein Variants

Protein Variants Comment Organism
additional information downregulation of the expression of an exo-beta-1,3-glucanase, exg2, using RNA interference, phenotype, overview Lentinula edodes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lentinan + H2O Lentinula edodes lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity ?
-
 ?
lentinan + H2O Lentinula edodes SR-1 lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
 gene exg2
-
Lentinula edodes SR-1
-
 gene exg2
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Lentinula edodes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Lentinula edodes 4-nitrophenol + N-acetyl-beta-D-glucosaminide
-
 ?
curdlan + H2O
-
 Lentinula edodes ?
-
 ?
curdlan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?
lentinan + H2O
-
 Lentinula edodes ?
-
 ?
lentinan + H2O lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity Lentinula edodes ?
-
 ?
lentinan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?
lentinan + H2O lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity Lentinula edodes SR-1 ?
-
 ?
additional information enzyme activity with different substrates in wild-type and mutant strains, overview Lentinula edodes ?
-
 ?
additional information enzyme activity with different substrates in wild-type and mutant strains, overview Lentinula edodes SR-1 ?
-
 ?
pustulan + H2O
-
 Lentinula edodes ?
-
 ?
pustulan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
EXG2
-
 Lentinula edodes
exo-beta-1,3-glucanase
-
 Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 assay at Lentinula edodes

General Information

General Information Comment Organism
malfunction the enzyme-downregulated strain shows significantly lower lentinan degrading activity (60-70% of the wild-type strain) in the fruiting bodies 2-4 days after harvesting. The lentinan content of fresh fruiting bodies is similar in the wild-type and EXG2 downregulated strains, but in the downregulated strain, only 25.4% of the lentinan is lost after 4 days, indicating that downregulation of EXG2 enables keeping the lentinan content high longer Lentinula edodes