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Literature summary for 3.2.1.58 extracted from

  • Hrmova, M.; Harvey, A.J.; Wang, J.; Shirley, N.J.; Jones, G.P.; Stone, B.A.; Hoj, P.B.; Fincher, G.B.
    Barley beta-D-glucan exohydrolases with beta-D-glucosidase activity (1996), J. Biol. Chem., 271, 5277-5286.
    View publication on PubMed
Search for more literature for 3.2.1.58

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-delta-lactone
-
 Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 4-nitrophenyl beta-D-glucoside
-
 Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 x * 69000, isoenzyme I, SDS-PAGE Hordeum vulgare
71000
-
 x * 71000, isoenzyme II, SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q42835
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I: 47fold, isoenzyme II: 128fold Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
 isoenzyme I Hordeum vulgare
12.8
-
 isoenzyme II Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Hordeum vulgare D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside
-
 Hordeum vulgare p-nitrophenol + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 69000, isoenzyme I, SDS-PAGE Hordeum vulgare
? x * 71000, isoenzyme II, SDS-PAGE Hordeum vulgare