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Literature summary for 3.2.1.58 extracted from

  • Kitamoto, Y.; Kono, R.; Shimotori, A.; Mori, N.; Ichikawa, Y.
    Purification and some properties of an exo-beta-1,3-glucanase from Trichoderma harzianum (1987), Agric. Biol. Chem., 51, 3385-3386.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Trichoderma harzianum
Hg2+
-
Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
laminaritetraose
-
Trichoderma harzianum
0.28
-
Laminaripentaose or 2.08 mg/ml Trichoderma harzianum
0.36
-
Laminarihexaose
-
Trichoderma harzianum
0.85
-
Laminarin molecular weight postulated to be 2448 Trichoderma harzianum
1.49
-
laminaritriose
-
Trichoderma harzianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
gel filtration Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trichoderma harzianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
593
-
-
Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
curdlan + H2O
-
Trichoderma harzianum ?
-
?
laminaribiose + H2O
-
Trichoderma harzianum D-glucose
-
?
laminarihexaose + H2O
-
Trichoderma harzianum D-glucose + laminarioligosaccharides
-
?
laminarin + H2O
-
Trichoderma harzianum D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
?
laminaripentaose + H2O
-
Trichoderma harzianum D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
?
laminaritetraose + H2O
-
Trichoderma harzianum D-glucose + laminaribiose + laminaritriose different combinations and amount of these products, depending on organism ?
laminaritriose + H2O
-
Trichoderma harzianum D-glucose + laminaribiose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
-
Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
Trichoderma harzianum