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Literature summary for 3.2.1.55 extracted from

  • Fujita, K.; Sakamoto, A.; Kaneko, S.; Kotake, T.; Tsumuraya, Y.; Kitahara, K.
    Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum (2019), Appl. Microbiol. Biotechnol., 103, 1299-1310 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme without the N-terminal signal peptide in Escherichia coli strain BL21(DE3) Bifidobacterium longum subsp. Longum

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits 47% at 1 mM Bifidobacterium longum subsp. Longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for larch type II arabinogalactan is 7.86 mg/ml for the recombinant enzyme Bifidobacterium longum subsp. Longum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 24% at 1 mM Bifidobacterium longum subsp. Longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. Longum A0A401ETL2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme without the N-terminal signal peptide from Escherichia coli strain BL21(DE3) Bifidobacterium longum subsp. Longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium longum subsp. Longum 4-nitrophenol + L-arabinofuranose
-
 ?
arabinan + H2O release of L-arabinofuranose Bifidobacterium longum subsp. Longum L-arabinofuranose + ?
-
 ?
beta-D-Galp-(1->6)-[alpha-L-Araf-(1->3)]-beta-D-Galp-(1->6)-beta-D-Galp + H2O recombinant BlArafA releases L-arabinose from the substrate, which is a beta-1,6-galactan side chain containing alpha-1,3-linked L-Araf Bifidobacterium longum subsp. Longum L-arabinofuranose + beta-D-Gal-(1->6)-beta-D-Gal-(1->6)-beta-D-Gal
-
 ?
gum arabic + H2O release of L-arabinofuranose, low activity Bifidobacterium longum subsp. Longum L-arabinofuranose + ?
-
 ?
larch type II arabinogalactan + H2O release of L-arabinofuranose, degradation profiles of type II AG Bifidobacterium longum subsp. Longum L-arabinofuranose + ?
-
 ?
methyl alpha-L-Araf-(1->2)-alpha-L-Araf + H2O low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf Bifidobacterium longum subsp. Longum L-arabinofuranose + L-Araf-alpha-O-methyl ether
-
 ?
methyl alpha-L-Araf-(1->3)-alpha-L-Araf + H2O
-
 Bifidobacterium longum subsp. Longum L-arabinofuranose + L-Araf-alpha-O-methyl ether
-
 ?
methyl alpha-L-Araf-(1->5)-alpha-L-Araf + H2O low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf Bifidobacterium longum subsp. Longum L-arabinofuranose + L-Araf-alpha-O-methyl ether
-
 ?
additional information the recombinant alpha-L-arabinofuranosidase releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview. The enzyme shows 100, 86.1, and 69.1% specificities for radish root AGP, larch AG, and arabinan, respectively. Arabinoxylan is a poor substrate. BlArafA is an alpha-L-arabinofuranosidase, which recognizes oligosaccharides containing alpha-1,3-linked L-Araf structures in type II AG and arabinan Bifidobacterium longum subsp. Longum ?
-
 ?
radish root arabinogalactan-protein + H2O release of L-arabinofuranose Bifidobacterium longum subsp. Longum L-arabinofuranose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 133000, recombinant His-tagged enzyme without signal peptide, SDS-PAGE, x * 132981, His-tagged enzyme without signal peptide, sequence calculation Bifidobacterium longum subsp. Longum

Synonyms

Synonyms Comment Organism
BlAraf
-
 Bifidobacterium longum subsp. Longum
blArafA
-
 Bifidobacterium longum subsp. Longum
GH43_22 alpha-L-arabinofuranosidase
-
 Bifidobacterium longum subsp. Longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Bifidobacterium longum subsp. Longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
220
-
 larch type II arabinogalactan pH 5.5, 40°C, recombinant enzyme Bifidobacterium longum subsp. Longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 recombinant enzyme Bifidobacterium longum subsp. Longum

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 5. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains Bifidobacterium longum subsp. Longum
metabolism GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) Bifidobacterium longum subsp. Longum
physiological function type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum Bifidobacterium longum subsp. Longum