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Literature summary for 3.2.1.55 extracted from

  • Phuengmaung, P.; Kunishige, Y.; Sukhumsirichart, W.; Sakamoto, T.
    Identification and characterization of GH62 bacterial alpha-L-arabinofuranosidase from thermotolerant Streptomyces sp. SWU10 that preferentially degrades branched L-arabinofuranoses in wheat arabinoxylan (2018), Enzyme Microb. Technol., 112, 22-28 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Streptomyces sp. SWU10

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 15% residual activity at 1 mM Streptomyces sp. SWU10
Cu2+ 18% residual activity at 1 mM Streptomyces sp. SWU10
EDTA 30% residual activity at 1 mM Streptomyces sp. SWU10
Fe2+ 22% residual activity at 1 mM Streptomyces sp. SWU10
Fe3+ 70% residual activity at 1 mM Streptomyces sp. SWU10
Hg2+ 59% residual activity at 1 mM Streptomyces sp. SWU10
Zn2+ 72% residual activity at 1 mM Streptomyces sp. SWU10

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 128% activity at 1 mM Streptomyces sp. SWU10
Ca2+ 125% activity at 1 mM Streptomyces sp. SWU10
Mn2+ 115% activity at 1 mM Streptomyces sp. SWU10
additional information not influenced by Na+, Ni2+, K+, and Co2+ Streptomyces sp. SWU10

Organism

Organism UniProt Comment Textmining
Streptomyces sp. SWU10 A0A2Z5VL17
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and Superdex 75 gel filtration Streptomyces sp. SWU10

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 cell-free extract, at pH 5.0 and 50°C Streptomyces sp. SWU10
1.29
-
 after 32.3fold purification, at pH 5.0 and 50°C Streptomyces sp. SWU10

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O
-
 Streptomyces sp. SWU10 alpha-L-arabinofuranose + ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside and 4-nitropheny beta-D-xylopyranoside Streptomyces sp. SWU10 ?
-
 ?
sugar beet arabinan + H2O
-
 Streptomyces sp. SWU10 alpha-L-arabinofuranose + ?
-
 ?
sugar beet debranched arabinan + H2O
-
 Streptomyces sp. SWU10 alpha-L-arabinofuranose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Streptomyces sp. SWU10 alpha-L-arabinofuranose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Streptomyces sp. SWU10
? x * 46232, calculated from amino acid sequence Streptomyces sp. SWU10

Synonyms

Synonyms Comment Organism
Abf62A
-
 Streptomyces sp. SWU10
arabinoxylan arabinofuranohydrolase
-
 Streptomyces sp. SWU10
AXH
-
 Streptomyces sp. SWU10
swu62A
-
 Streptomyces sp. SWU10

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces sp. SWU10

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 about 55% activity at 30°C, about 70% activity at 40°C, 100% activity at 50°C, about 45% activity at 60°C Streptomyces sp. SWU10

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 the enzyme loses all activity after 1 h at 60°C. 60% activity remain after 1 h at 20-50°C Streptomyces sp. SWU10

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Streptomyces sp. SWU10

pH Range

pH Minimum pH Maximum Comment Organism
4 7 more than 60% activity between pH 4.0 and 7.0 Streptomyces sp. SWU10

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.1 10.5 more than 70% of the initial enzymatic activity remain after 1 h at pH 4.1-10.0 Streptomyces sp. SWU10

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. SWU10 calculated from amino acid sequence
-
 6.1