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Literature summary for 3.2.1.55 extracted from

  • Wang, W.; Andric, N.; Sarch, C.; Silva, B.T.; Tenkanen, M.; Master, E.R.
    Constructing arabinofuranosidases for dual arabinoxylan debranching activity (2018), Biotechnol. Bioeng., 115, 41-49 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Streptomyces thermoviolaceus
expressed in Escherichia coli BL21(lambdaDE3) cells Bifidobacterium adolescentis

Protein Variants

Protein Variants Comment Organism
R239A the activity of the mutant enzyme with wheat arabinoxylan is reduced by 100times compared to the wild type enzyme Streptomyces thermoviolaceus
R239A/F212A the activity of the mutant enzyme with wheat arabinoxylan is reduced by 200times compared to the wild type enzyme Streptomyces thermoviolaceus
R239A/F212A/I233A the activity of the mutant enzyme with wheat arabinoxylan is reduced by 8000times compared to the wild type enzyme Streptomyces thermoviolaceus
R239A/I233A the activity of the mutant enzyme with wheat arabinoxylan is reduced by 250times compared to the wild type enzyme Streptomyces thermoviolaceus
R239K the activity of the mutant enzyme with wheat arabinoxylan is reduced by 100times compared to the wild type enzyme Streptomyces thermoviolaceus
R239S the activity of the mutant enzyme with wheat arabinoxylan is reduced by 150times compared to the wild type enzyme Streptomyces thermoviolaceus

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis Q5JB56
-
-
Bifidobacterium adolescentis ATCC 15703 Q5JB56
-
-
Streptomyces thermoviolaceus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Uno-Q column chromatography Streptomyces thermoviolaceus
Ni-NTA resin column chromatography and Uno-Q column chromatography Bifidobacterium adolescentis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
di-2,3-arabinoxylan + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
di-2,3-arabinoxylan + H2O
-
 Bifidobacterium adolescentis ATCC 15703 ?
-
 ?
mono-2,3-arabinoxylan + H2O
-
 Streptomyces thermoviolaceus ?
-
 ?
additional information no activity with di-2,3-arabinoxylan Streptomyces thermoviolaceus ?
-
 ?
additional information no activity with mono-2,3-arabinoxylan Bifidobacterium adolescentis ?
-
 ?
additional information no activity with mono-2,3-arabinoxylan Bifidobacterium adolescentis ATCC 15703 ?
-
 ?
wheat arabinoxylan + H2O
-
 Streptomyces thermoviolaceus alpha-L-arabinofuranose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Bifidobacterium adolescentis alpha-L-arabinofuranose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Bifidobacterium adolescentis ATCC 15703 alpha-L-arabinofuranose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35900, calculated from amino acid sequence Streptomyces thermoviolaceus
? x * 59300, calculated from amino acid sequence Bifidobacterium adolescentis

Synonyms

Synonyms Comment Organism
Abf62A
-
 Streptomyces thermoviolaceus
Abf62A
-
 Bifidobacterium adolescentis
alpha-L-arabinofuranosidase
-
 Streptomyces thermoviolaceus
alpha-L-arabinofuranosidase
-
 Bifidobacterium adolescentis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme retains 0.03% activity after 1 h of incubation at 55°C Bifidobacterium adolescentis
55
-
 the enzyme retains 73% activity after 1 h of incubation at 55°C Streptomyces thermoviolaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bifidobacterium adolescentis
7
-
-
 Streptomyces thermoviolaceus