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Literature summary for 3.2.1.55 extracted from
- Improved activity of alpha-L-arabinofuranosidase from Geobacillus vulcani GS90 by directed evolution Investigation on thermal and alkaline stability (2019), Biotechnol. Appl. Biochem., 66, 101-107 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(lambdaDE3) cells | Geobacillus vulcani |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L307S | the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme | Geobacillus vulcani |
| Q90H/L307S | the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme | Geobacillus vulcani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus vulcani | - |
- |
- |
| Geobacillus vulcani GS90 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tag nickel affinity column chromatography | Geobacillus vulcani |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 72.066 | - |
wild type enzyme, at pH 5.0 and 70°C | Geobacillus vulcani |
| 142.024 | - |
mutant enzyme Q90H/L307S, at pH 5.0 and 70°C | Geobacillus vulcani |
| 160.991 | - |
mutant enzyme L307S, at pH 5.0 and 70°C | Geobacillus vulcani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus vulcani | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |  |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus vulcani GS90 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Geobacillus vulcani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABF | - |
Geobacillus vulcani |
| alpha-L-arabinofuranosidase | - |
Geobacillus vulcani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus vulcani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 72 | the wild type enzyme retains about 20% activity after 100 min at 70°C, about 20% activity after 40 min at 71°C, and less than 5% activity after 30 min at 72°C | Geobacillus vulcani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Geobacillus vulcani |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.6 | the wild type enzyme retains about 20% activity after 100 min at pH 5.0 and 70°C and about 30% activity after 80 min at pH 9.6 and 70°C | Geobacillus vulcani |
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Release 2023.1 (January 2023)
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