Literature summary for 3.2.1.55 extracted from

Saleh, M.A.; Han, W.J.; Lu, M.; Wang, B.; Li, H.; Kelly, R.M.; Li, F.L.
Two Distinct alpha-L-arabinofuranosidases in Caldicellulosiruptor species drive degradation of arabinose-based polysaccharides (2017), Appl. Environ. Microbiol., 83, e00574-17 .

Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Caldicellulosiruptor sp. F32

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	39% residual activity at 5 mM	Caldicellulosiruptor sp. F32
Cu2+	0.8% residual activity at 1 mM	Caldicellulosiruptor sp. F32
EDTA	the enzyme retains 24% activity in the presence of 5 mM EDTA	Caldicellulosiruptor sp. F32
Fe2+	16% residual activity at 1 mM	Caldicellulosiruptor sp. F32
K+	14% residual activity at 1 mM	Caldicellulosiruptor sp. F32
Mg2+	76% residual activity at 5 mM	Caldicellulosiruptor sp. F32
Ni2+	82% residual activity at 1 mM	Caldicellulosiruptor sp. F32
SDS	the enzyme is inactive in the presence of 5 mM SDS	Caldicellulosiruptor sp. F32

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	4-nitrophenyl alpha-L-arabinofuranoside	at pH 6.0 and 80°C	Caldicellulosiruptor sp. F32

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Caldicellulosiruptor sp. F32	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	106% activity at 5 mM	Caldicellulosiruptor sp. F32
Mn2+	117% activity at 5 mM	Caldicellulosiruptor sp. F32

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sugar beet arabinan + H2O	Caldicellulosiruptor sp. F32	strong hydrolytic activity	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Caldicellulosiruptor sp. F32	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Caldicellulosiruptor sp. F32	4-nitrophenol + alpha-L-arabinofuranose	-	?
sugar beet arabinan + H2O	strong hydrolytic activity	Caldicellulosiruptor sp. F32	?	-	?

Synonyms

Synonyms	Comment	Organism
AbF51	-	Caldicellulosiruptor sp. F32
alpha-L-arabinofuranosidase	-	Caldicellulosiruptor sp. F32

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	-	Caldicellulosiruptor sp. F32

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	75	the half-lives of the enzyme at 70°C and 75°C are 100 h and 90 h, respectively	Caldicellulosiruptor sp. F32

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3900	-	4-nitrophenyl alpha-L-arabinofuranoside	at pH 6.0 and 80°C	Caldicellulosiruptor sp. F32

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Caldicellulosiruptor sp. F32

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6500	-	4-nitrophenyl alpha-L-arabinofuranoside	at pH 6.0 and 80°C	Caldicellulosiruptor sp. F32

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)