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Literature summary for 3.2.1.55 extracted from

  • Perez, R.; Eyzaguirre, J.
    Aspergillus fumigatus produces two arabinofuranosidases from glycosyl hydrolase family 62 comparative properties of the recombinant enzymes (2016), Appl. Biochem. Biotechnol., 179, 143-154 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.9
-
 4-nitrophenyl alpha-L-arabinofuranoside isoform AbfII, at pH 5.0 and 42°C Aspergillus fumigatus
94.2
-
 4-nitrophenyl alpha-L-arabinofuranoside isoform AbfI, at pH 5.0 and 37°C Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
sulfopropyl Sepharose column chromatography (isoform AbfI) and hydroxyapatite column chromatography (isoform AbfII) Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + H2O isoform AbfII exhibits very low activity with this substrate Aspergillus fumigatus 4-nitrophenol + alpha-D-galactopyranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O 100% activity Aspergillus fumigatus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O very low activity Aspergillus fumigatus 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O very low activity Aspergillus fumigatus 4-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl beta-D-glucofuranoside + H2O very low activity Aspergillus fumigatus 4-nitrophenol + beta-D-glucofuranose
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O isoform AbfII exhibits very low activity with this substrate Aspergillus fumigatus 4-nitrophenol + beta-D-mannopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O isoform AbfII exhibits very low activity with this substrate Aspergillus fumigatus 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-L-fucopyranoside + H2O very low activity Aspergillus fumigatus 4-nitrophenol + beta-L-fucopyranose
-
 ?
4-nitrophenyl beta-L-galactofuranoside + H2O very low activity Aspergillus fumigatus 4-nitrophenol + beta-L-galactofuranose
-
 ?
additional information isoform AbfI shows no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl beta-D-xylopyranoside Aspergillus fumigatus ?
-
 ?
additional information isoforms AbfI and AbfII are inactive towards debranched arabinan Aspergillus fumigatus ?
-
 ?
rye arabinoxylan + H2O
-
 Aspergillus fumigatus ?
-
 ?
sugar beet pulp arabinan + H2O substrate for isoform AbfII Aspergillus fumigatus ?
-
 ?
wheat arabinoxylan + H2O
-
 Aspergillus fumigatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34700, isoform AbfI, calculated from amino acid sequence Aspergillus fumigatus
? x * 41300, isoform AbfII, calculated from amino acid sequence Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
AbfI isoform Aspergillus fumigatus
AbfII isoform Aspergillus fumigatus
alpha-L-arabinofuranosidase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 isoform AbfI Aspergillus fumigatus
42
-
 isoform AbfII Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Aspergillus fumigatus