Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Halothermothrix orenii |
Crystallization (Comment) | Organism |
---|---|
structure at 1.10 A resolution. As for other GH43 members, the enzyme folds as a five-bladed -propeller. The structure features a metal-binding site on the propeller axis, near the active site | Halothermothrix orenii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilization | Halothermothrix orenii | |
Mg2+ | stabilization | Halothermothrix orenii | |
Mn2+ | pH 7.0, adding 2 mM Mn2+ increases the melting temperature of the enzyme from 63°C to 68.5°C | Halothermothrix orenii | |
additional information | divalent cation is required | Halothermothrix orenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halothermothrix orenii | B8CZV1 | - |
- |
Halothermothrix orenii DSM 9562 | B8CZV1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
presence of 4 M NaCl and Mg2+, pH 6.5, 50°C | Halothermothrix orenii |
0.036 | - |
presence of 4 M NaCl and Mn2+, pH 6.5, 50°C | Halothermothrix orenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Halothermothrix orenii | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Halothermothrix orenii DSM 9562 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AraF43 | - |
Halothermothrix orenii |
Hore_20580 | - |
Halothermothrix orenii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
melting temperature | Halothermothrix orenii |
68.5 | - |
melting temperatue, presence of 2 mM Mn2+ | Halothermothrix orenii |
76 | - |
melting temperature, presence of 4 M NaCl and Mn2+ at pH 6.5 | Halothermothrix orenii |