Cloned (Comment) | Organism |
---|---|
gene CsAra, DNA and amino acid sequence determination and analysis, sequence comparisons | Chaetomium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.43 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | native enzyme, pH 5.0, 50°C | Chaetomium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Chaetomium sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51600 | - |
gel filtration | Chaetomium sp. |
52900 | - |
1 * 52900, SDS-PAGE | Chaetomium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chaetomium sp. | K7QK60 | gene CsAra | - |
Chaetomium sp. CQ31 | K7QK60 | gene CsAra | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 1030fold to homogeneity | Chaetomium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.6 | - |
purified native enzyme, pH 5.0, 50°C | Chaetomium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Chaetomium sp. | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Chaetomium sp. CQ31 | 4-nitrophenol + L-arabinofuranose | - |
? | |
additional information | the enzyme shows strict substrate specificity for the alpha-L-arabinofuranosyl linkage | Chaetomium sp. | ? | - |
? | |
additional information | the enzyme shows strict substrate specificity for the alpha-L-arabinofuranosyl linkage | Chaetomium sp. CQ31 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52900, SDS-PAGE | Chaetomium sp. |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-Afase | - |
Chaetomium sp. |
alpha-L-arabinofuranosidase | - |
Chaetomium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Chaetomium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 85 | activity range, profile overview | Chaetomium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | purified native enzyme, stable up to 55°C for 30 min, half-lives at 55°C, 60°C, 65°C and 70°C are 167.4 min, 104.6 min, 28.1 min and 10.3 min, respectively | Chaetomium sp. |
60 | - |
purified native enzyme, 1 h, 75% activity remaining | Chaetomium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Chaetomium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, broad optimal activity ranging with high activity at pH 4.5-7.0, the enzyme activity decreases sharply when the pH increases above pH 8.0 or decreased below pH 4.0, profile overview | Chaetomium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified native enzyme, completely stable at | Chaetomium sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to glycosyl hydrolase family 43, GH43 | Chaetomium sp. |