Literature summary for 3.2.1.55 extracted from

Ahmed, S.; Luis, A.S.; Bras, J.L.; Ghosh, A.; Gautam, S.; Gupta, M.N.; Fontes, C.M.; Goyal, A.
A novel alpha-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum (2013), PLoS ONE, 8, e73575.

Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment)	Organism
functional recombinant expression of full-length enzyme and isolated catalytic module CtGH43 in Escherichia coli strain BL21(DE3) pLysS	Acetivibrio thermocellus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	80% inhibition at 20 mM	Acetivibrio thermocellus
Al3+	50% inhibition at 20 mM	Acetivibrio thermocellus
Co2+	75% inhibition at 20 mM	Acetivibrio thermocellus
Cu2+	70% inhibition at 10 mM	Acetivibrio thermocellus
EDTA	95% inhibition at 20 mM	Acetivibrio thermocellus
EGTA	95% inhibition at 20 mM	Acetivibrio thermocellus
Fe3+	50% inhibition at 20 mM	Acetivibrio thermocellus
Hg2+	80% inhibition at 20 mM	Acetivibrio thermocellus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	4-nitrophenyl-alpha-L-arabinofuranoside	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.05	-	4-nitrophenyl-alpha-L-arabinofuranoside	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.072	-	soluble wheat arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.078	-	soluble wheat arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.08	-	oat spelt xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.08	-	rye arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.082	-	rye arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.085	-	oat spelt xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.09	-	insoluble wheat arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.093	-	4-nitrophenyl-alpha-L-arabinopyranoside	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.097	-	4-nitrophenyl-alpha-L-arabinopyranoside	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.1	-	insoluble wheat arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.7	-	beechwood xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.8	-	beechwood xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.9	-	birchwood xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.95	-	birchwood xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates the enzyme 2.16fold at 8 mM, presence of Ca2+ ions imparts thermal stability	Acetivibrio thermocellus
Mg2+	activates the enzyme 2.1fold at 6 mM	Acetivibrio thermocellus
Mn2+	activates the enzyme 1.2fold at 4 mM	Acetivibrio thermocellus
Ni2+	activates the enzyme 1.2fold at 4 mM	Acetivibrio thermocellus
Zn2+	activates the enzyme 1.2fold at 2 mM	Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE	Acetivibrio thermocellus
63000	-	x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	A3DEX4	two isozymes	-
Acetivibrio thermocellus ATCC 27405	A3DEX4	two isozymes	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1	-	recombinant full-length enzyme, pH 5.7, 50°C, substrate insoluble wheat arabinoxylan	Acetivibrio thermocellus
1.2	-	recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate insoluble wheat arabinoxylan	Acetivibrio thermocellus
1.7	-	recombinant full-length enzyme, pH 5.7, 50°C, substrate oat spelt xylan	Acetivibrio thermocellus
1.8	-	recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate oat spelt xylan	Acetivibrio thermocellus
2.5	-	recombinant full-length enzyme, pH 5.7, 50°C, substrate soluble wheat arabinoxylan	Acetivibrio thermocellus
2.7	-	recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate soluble wheat arabinoxylan	Acetivibrio thermocellus
4.7	-	recombinant full-length enzyme, pH 5.7, 50°C, substrate rye arabinoxylan	Acetivibrio thermocellus
5	-	recombinant catalytic module CtGH43, pH 5.7, 50°C, substrate rye arabinoxylan	Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Acetivibrio thermocellus	4-nitrophenol + L-arabinofuranose	-	?
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Acetivibrio thermocellus ATCC 27405	4-nitrophenol + L-arabinofuranose	-	?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O	-	Acetivibrio thermocellus	4-nitrophenol + L-arabinopyranose	-	?
beechwood xylan + H2O	-	Acetivibrio thermocellus	?	-	?
beechwood xylan + H2O	-	Acetivibrio thermocellus ATCC 27405	?	-	?
birchwood xylan + H2O	-	Acetivibrio thermocellus	?	-	?
birchwood xylan + H2O	-	Acetivibrio thermocellus ATCC 27405	?	-	?
insoluble wheat arabinoxylan + H2O	-	Acetivibrio thermocellus	?	-	?
additional information	good substrates are rye and wheat arabinoxylan and oat spelt xylan with release of L-arabinose. Lower activity with xylan from beechwood and birchwood, arabinogalactan, arabinan from sugar beet, carboxy methyl cellulose and carboxy ethylcellulose	Acetivibrio thermocellus	?	-	?
additional information	good substrates are rye and wheat arabinoxylan and oat spelt xylan with release of L-arabinose. Lower activity with xylan from beechwood and birchwood, arabinogalactan, arabinan from sugar beet, carboxy methyl cellulose and carboxy ethylcellulose	Acetivibrio thermocellus ATCC 27405	?	-	?
oat spelt xylan + H2O	-	Acetivibrio thermocellus	?	-	?
oat spelt xylan + H2O	-	Acetivibrio thermocellus ATCC 27405	?	-	?
rye arabinoxylan + H2O	-	Acetivibrio thermocellus	?	-	?
soluble wheat arabinoxylan + H2O	-	Acetivibrio thermocellus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 63000, recombinant full-length enzyme, SDS-PAGE, x * 34000, recombinant catalytic module CtGH43 , SDS-PAGE	Acetivibrio thermocellus
More	the enzyme displays an N-terminal catalytic module CtGH43 followed by two carbohydrate binding modules CtCBM6A and CtCBM6B towards the C-terminus. Circular dichroism analysis of catalytic domain CtGH43 shows 48% beta-sheets, 49% random coils but only 3% alpha-helices	Acetivibrio thermocellus

Synonyms

Synonyms	Comment	Organism
Ct43Araf	-	Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.43	-	beechwood xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
0.45	-	birchwood xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.47	-	beechwood xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
0.48	-	birchwood xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
1.02	-	insoluble wheat arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
1.05	-	insoluble wheat arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
1.08	-	oat spelt xylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
1.12	-	oat spelt xylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
3.2	-	soluble wheat arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
3.48	-	soluble wheat arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
3.5	-	4-nitrophenyl-alpha-L-arabinopyranoside	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
3.53	-	4-nitrophenyl-alpha-L-arabinopyranoside	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
4.7	-	rye arabinoxylan	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
4.72	-	4-nitrophenyl-alpha-L-arabinofuranoside	pH 5.7, 50°C, recombinant full-length enzyme	Acetivibrio thermocellus
4.78	-	4-nitrophenyl-alpha-L-arabinofuranoside	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus
4.97	-	rye arabinoxylan	pH 5.7, 50°C, recombinant catalytic module CtGH43	Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.4	-	recombinant catalytic module CtGH43	Acetivibrio thermocellus
5.7	-	recombinant full-length enzyme	Acetivibrio thermocellus

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the family 43 glycoside hydrolase, GH43	Acetivibrio thermocellus
additional information	the enzyme displays an N-terminal catalytic module CtGH43 followed by two carbohydrate binding modules CtCBM6A and CtCBM6B towards the C-terminus, CtCBM6A and CtCBM6B do not have any effect on the enzyme activity	Acetivibrio thermocellus

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Release 2023.1 (January 2023)