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Literature summary for 3.2.1.55 extracted from
- Cloning, expression, and characterization of a thermostable GH51 alpha-L-arabinofuranosidase from Paenibacillus sp. DG-22 (2014), J. Microbiol. Biotechnol., 24, 236-244.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Paenibacillus sp. |  |
| DTT | - |
Paenibacillus sp. | |
| EDTA | - |
Paenibacillus sp. | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene abfA, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain M15(pREP4), subcloning in Escherichia coli strain DH5alpha | Paenibacillus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | almost complete inhibition at 1 mM | Paenibacillus sp. | |
| Ca2+ | 83% inhibition at 1 mM, 99.5% at 10 mM | Paenibacillus sp. | |
| Co2+ | almost complete inhibition at 1 mM | Paenibacillus sp. | |
| Cu2+ | almost complete inhibition at 1 mM | Paenibacillus sp. | |
| Fe2+ | 44% inhibition at 1 mM, 95% at 10 mM | Paenibacillus sp. | |
| Hg2+ | complete inhibition at 1 mM | Paenibacillus sp. | |
| K+ | 66% inhibition at 1 mM, 95% at 10 mM | Paenibacillus sp. | |
| Mg2+ | 56% inhibition at 1 mM, 70% at 10 mM | Paenibacillus sp. | |
| Mn2+ | no inhibition at 1 mM, 44% at 10 mM | Paenibacillus sp. | |
| Na+ | slight inhibition at 1-10 mM | Paenibacillus sp. | |
| Rb+ | 6% inhibition at 1 mM, 30% at 10 mM | Paenibacillus sp. | |
| SDS | 71% inhibition at 1 mM, 96% at 10 mM | Paenibacillus sp. | |
| Zn2+ | 96% inhibition at 1 mM, almost complete at 10 mM | Paenibacillus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | recombinant His6-tagged enzyme, pH 6.0, 60°C | Paenibacillus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on activity with Mn2+, Na+, and Rb+ | Paenibacillus sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56520 | - |
4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
| 57000 | - |
4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
| 221500 | - |
recombinant His-tagged enzyme, gel filtration | Paenibacillus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Paenibacillus sp. | the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans | ? | - |
? | |
| additional information | Paenibacillus sp. DG-22 | the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus sp. | A0A075EDT8 | gene abfA | - |
| Paenibacillus sp. DG-22 | A0A075EDT8 | gene abfA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme 7.4fold from Escherichia coli by nickel affinity chromatography to homogeneity | Paenibacillus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 96.7 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 60°C | Paenibacillus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus sp. | 4-methylumbelliferol + alpha-L-arabinofuranose | - |
? | |
| 4-methylumbelliferyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus sp. DG-22 | 4-methylumbelliferol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus sp. | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus sp. DG-22 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| additional information | the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans | Paenibacillus sp. | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with p-nitrophenyl beta-D-xylopyranoside | Paenibacillus sp. | ? | - |
? | |
| additional information | the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans | Paenibacillus sp. DG-22 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with p-nitrophenyl beta-D-xylopyranoside | Paenibacillus sp. DG-22 | ? | - |
? | |
| oat spelt arabinoxylan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
| oat spelt arabinoxylan + H2O | - |
Paenibacillus sp. DG-22 | ? | - |
? | |
| wheat L-arabinoxylan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AFase | - |
Paenibacillus sp. |
| alpha-L-arabinofuranosidase | - |
Paenibacillus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Paenibacillus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | activity range, profile overview | Paenibacillus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Paenibacillus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range, profile overview | Paenibacillus sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | purified recombinant enzyme, over 80% activity remaining | Paenibacillus sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the family 51 glycoside hydrolase, GH51 | Paenibacillus sp. |
| metabolism | the presence of L-arabinofuranoside side chains restricts the action of endo-acting xylanases. Therefore, the AFases are the most important accessory enzymes involved in synergy with other hemicellulases for the complete hydrolysis of hemicelluloses | Paenibacillus sp. |
| additional information | the recombinant enzyme shows a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan | Paenibacillus sp. |
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