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Literature summary for 3.2.1.55 extracted from
- Production of ginsenoside Rd from ginsenoside Rc by alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (2013), J. Microbiol. Biotechnol., 23, 483-488.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant effect by 5-10% v/v toluene | Caldicellulosiruptor saccharolyticus | |
| n-hexane | activates about 20% at 5-10% v/v, and 230% at 30% v/v | Caldicellulosiruptor saccharolyticus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the thermostable enzyme is used for production of ginsenoside Rd from ginsenoside Rc, optimal reaction conditions are pH 5.5, 80°C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% v/v n-hexane | Caldicellulosiruptor saccharolyticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the enzyme in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-methyl-1-butanol | inhibits 80% and 97% at 5% and 10% v/v, respectively | Caldicellulosiruptor saccharolyticus | |
| acetone | inhibits 15% and 50% at 5% and 10% v/v, respectively | Caldicellulosiruptor saccharolyticus | |
| DMSO | inhibits 10% and 40% at 5% and 10% v/v, respectively | Caldicellulosiruptor saccharolyticus | |
| ethanol | inhibits 25% and 55% at 5% and 10% v/v, respectively | Caldicellulosiruptor saccharolyticus | |
| methanol | inhibits 15% and 50% at 5% and 10% v/v, respectively | Caldicellulosiruptor saccharolyticus | |
| additional information | no significant effect by 5-10% v/v toluene | Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57882 | - |
8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 58000 | - |
8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 460000 | - |
recombinant enzyme, gel filtration | Caldicellulosiruptor saccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | A4XJR8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 25.6fold from Escherichia coli by heat treatment and anion exchange chromatography | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 75.7 | - |
purified recombinant enzyme, pH 5.5, 85°C | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + L-arabinofuranose | - |
? | |
| ginsenoside Mc + H2O | - |
Caldicellulosiruptor saccharolyticus | ginsenoside K + L-arabinofuranose | - |
? | |
| ginsenoside Rc + H2O | thermostable enzyme produces ginsenoside Rd from ginsenoside Rc, optimal reaction conditions are pH 5.5, 80°C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% v/v n-hexane. The enzyme completely converts ginsenoside Rc to ginsenoside Rd with very high productivity | Caldicellulosiruptor saccharolyticus | ginsenoside Rd + L-arabinofuranose | - |
? | |
| additional information | substrate specificity, overview. No activity with 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-L-arabinofuranoside, 4-nitrophenyl-beta-L-arabinopyranoside 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, ginsenoside Rb2, which contains alpha-L-arabinopyranoside at C-20, or ginsenoside Rb1, which contains beta-D-glucopyranoside at C-20 | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 57882, sequence calculation, 8 * 58000, recombinant enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | over 40% activity within this range, profile overview | Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 85 | thermal inactivation of the purified enzyme follows first-order kinetics with half-lives of 130, 41, 2.5, and 0.12 h at 70°C, 75°C, 80°C, and 85°C, respectively | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | activity range, profile overview | Caldicellulosiruptor saccharolyticus |
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