Cloned (Comment) | Organism |
---|---|
isozymes PfABF62a, PfABF62b, and PfABF62c, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM in Pichia pastoris strain GS115, subcloning in Escherichia coli | Talaromyces funiculosus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an isozyme pfabf62c mutant pfabf62cDELTACBM truncated of the DNA region encoding canonical cellulose binding module, CBM1 | Talaromyces funiculosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
0.47 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62b | Talaromyces funiculosus | |
0.58 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
1 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
1.4 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
2.7 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus | |
8.2 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the isozymes are secreted | Talaromyces funiculosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35400 | - |
x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation | Talaromyces funiculosus |
37100 | - |
x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation | Talaromyces funiculosus |
37400 | - |
x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation | Talaromyces funiculosus |
41300 | - |
x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation | Talaromyces funiculosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces funiculosus | - |
three isozymes PfABF62a, PfABF62b, and PfABF62c | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM from Pichia pastoris strain GS115 by immobilized metal affinity chromatography | Talaromyces funiculosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose | mode of action of Penicillium funiculosum family 62 isozymes, overview | Talaromyces funiculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinan + H2O | - |
Talaromyces funiculosus | ? | - |
? | |
additional information | no activity with other polysaccharides or polymers than arabinan and arabinoxylan | Talaromyces funiculosus | ? | - |
? | |
rye arabinoxylan + H2O | enzymatic fingerprinting analysis by high performance anion exchange chromatogra-phy with pulsed amperometric detection | Talaromyces funiculosus | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble wheat arabinoxylans, enzymatic fingerprinting analysis by high performance anion exchange chromatogra-phy with pulsed amperometric detection | Talaromyces funiculosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation | Talaromyces funiculosus |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-Afase | - |
Talaromyces funiculosus |
alpha-L-arabinofuranosidase | - |
Talaromyces funiculosus |
PfABF62a | - |
Talaromyces funiculosus |
PfABF62b | - |
Talaromyces funiculosus |
PfABF62c | - |
Talaromyces funiculosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isozymes PfABF62a and PfABF62b | Talaromyces funiculosus |
50 | - |
isozyme PfABF62c | Talaromyces funiculosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
0.85 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
0.925 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus | |
0.96 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
0.99 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus | |
1.11 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
1.2 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62b | Talaromyces funiculosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.6 | - |
isozyme PfABF62a | Talaromyces funiculosus |
2.8 | - |
isozyme PfABF62c | Talaromyces funiculosus |
4.5 | - |
isozyme PfABF62b | Talaromyces funiculosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Talaromyces funiculosus | isozyme PfABF62a, sequence calculation | - |
4.66 |
Talaromyces funiculosus | isozyme PfABF62c, sequence calculation | - |
4.7 |
Talaromyces funiculosus | isozyme mutant pfabf62cDELTACBM, sequence calculation | - |
4.71 |
Talaromyces funiculosus | isozyme PfABF62b, sequence calculation | - |
5.24 |
General Information | Comment | Organism |
---|---|---|
evolution | the isozymes belong to the family 62 glycoside hydrolase, GH62, sequence comparisons, only isozyme PfABF62c is unique in bearing at its N-terminus a canonical family 1 carbohydrate-binding module of 37 amino acids length, which helps the protein to bind to microcrystalline cellulose | Talaromyces funiculosus |
physiological function | the enzyme participates in the hydrolysis of arabinoxylans in corn/wheat fibers used in poultry feed | Talaromyces funiculosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.121 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus | |
0.35 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62a | Talaromyces funiculosus | |
0.64 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
0.92 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
1.91 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme PfABF62c | Talaromyces funiculosus | |
2.425 | - |
rye arabinoxylan | pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM | Talaromyces funiculosus | |
2.56 | - |
wheat arabinoxylan | pH 4.0, 40°C, isozyme PfABF62b | Talaromyces funiculosus |