Cloned (Comment) | Organism |
---|---|
truncated 903 bp gene encoding the N-terminus of CtGH43 is part of a full-length family 43 glycoside hydrolase Ct43Araf which also contains two tandem family 6 carbohydrate-binding modules, CtCBM6A (405 bp) and CtCBM6B (402 bp), at the C-terminus, recombinant expression of the His6-tagged truncated enzyme in Escherichia coli strain BL21(DE3) pLysS | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged truncated enzyme, sitting drop vapour diffusion method, mixing of 120 mg/ml protein in 50 mM HEPES, pH 7.5, 200 mM NaCl, and 5 mM CaCl2 with precipitant solution containing 0.1 M sodium acetate pH 4.5, 2 M ammonium sulfate and (b) 0.1 M Tris, pH 8.5, 20% PEG 2000 monomethyl ether, 0.2 M trimethyl N-oxide for brick shaped crystals or containing 0.1 M Tris, pH 8.5, 20% PEG 2000 monomethyl ether, 0.2 M trimethyl N-oxide for monoclinic crystals, X-ray diffraction structure determination and analysis at resolution of 1.65 A for the cubic form and 1.1 A for the monoclinic form, molecular replacement method | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35860 | - |
x * 35860, recombinant His6-tagged truncated enzyme, SDS-PAGE | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acetivibrio thermocellus | the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans | ? | - |
? | |
additional information | Acetivibrio thermocellus ATCC 27405 | the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DIH0 | - |
- |
Acetivibrio thermocellus ATCC 27405 | A3DIH0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged truncated enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and gel filtration | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans | Acetivibrio thermocellus | ? | - |
? | |
additional information | the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans | Acetivibrio thermocellus ATCC 27405 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35860, recombinant His6-tagged truncated enzyme, SDS-PAGE | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Acetivibrio thermocellus |
alpha-L-Araf | - |
Acetivibrio thermocellus |
CtGH43 | - |
Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 43, GH43 | Acetivibrio thermocellus |