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Literature summary for 3.2.1.55 extracted from

  • Zhou, J.; Bao, L.; Chang, L.; Zhou, Y.; Lu, H.
    Biochemical and kinetic characterization of GH43 beta-D-xylosidase/alpha-L-arabinofuranosidase and GH30 alpha-L-arabinofuranosidase/beta-D-xylosidase from rumen metagenome (2012), J. Ind. Microbiol. Biotechnol., 39, 143-152.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured rumen bacterium

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl alpha-L-arabinofuranoside
-
uncultured rumen bacterium
4-nitrophenyl beta-D-xylopyranoside
-
uncultured rumen bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.23
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE uncultured rumen bacterium

Organism

Organism UniProt Comment Textmining
uncultured rumen bacterium E3TBJ3 from rumen of Bos grunniens
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography uncultured rumen bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
uncultured rumen bacterium 4-nitrophenol + alpha-L-arabinofuranose
-
?
additional information no activity on 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, rice straw xylan or birchwood xylan uncultured rumen bacterium ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE uncultured rumen bacterium

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
uncultured rumen bacterium
beta-D-xylosidase/alpha-L-arabinofuranosidase
-
uncultured rumen bacterium
RuXyn1
-
uncultured rumen bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme is relatively stable at 40°C, but loses 76% of its activity after 10 min at 45°C, and 97% of activity at 50°C. The irreversible thermal denaturation half-time of the enzyme is calculated to be 112 min at 40°C, and 5.8 min at 45°C uncultured rumen bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
uncultured rumen bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4 8 the enzyme retains over 72% of optimal activity at pH 6.0, at least 40% at pH 5.0, and more than 10% at pH 4.0, however enzyme activity decreases dramatically at pH 8.0, and only 20% of its activity remains uncultured rumen bacterium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
20.8
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.7
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium