Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.55 extracted from

  • Fujimoto, Z.; Ichinose, H.; Maehara, T.; Honda, M.; Kitaoka, M.; Kaneko, S.
    Crystal structure of an exo-1,5-alpha-L-arabinofuranosidase from Streptomyces avermitilis provides insights into the mechanism of substrate discrimination between exo- and endo-type enzymes in glycoside hydrolase family 43 (2010), J. Biol. Chem., 285, 34134-34143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21gold (DE3) cells Streptomyces avermitilis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.8 M sodium citrate, 0.2 M sodium chloride, and 0.1 M Tris, pH 7.0 Streptomyces avermitilis

Protein Variants

Protein Variants Comment Organism
D135A inactive Streptomyces avermitilis
D135N inactive Streptomyces avermitilis
D20A inactive Streptomyces avermitilis
D20N the mutant shows 0.7% of wild type activity Streptomyces avermitilis
E196A inactive Streptomyces avermitilis
L289A the mutant shows 10% of wild type activity Streptomyces avermitilis
N159A the mutant shows 1.5fold increased activity compared to the wild type Streptomyces avermitilis
N159L the mutant shows 19fold increased activity compared to the wild type Streptomyces avermitilis
Y192A the mutant shows 40% of wild type activity Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.5
-
4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
1.1
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
1.7
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
11.5
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
40.7
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces avermitilis 4-nitrophenol + alpha-L-arabinofuranose
-
?
alpha-1,5-L-arabinofuranobiose + H2O
-
Streptomyces avermitilis L-arabinofuranose
-
?
additional information the enzyme shows no activity towards alpha-1,2-L-arabinofuranobiose and alpha-1,3-L-arabinofuranobiose Streptomyces avermitilis ?
-
?

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
Streptomyces avermitilis
Araf43A
-
Streptomyces avermitilis
exo-1,5-alpha-L-arabinofuranosidase
-
Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.11
-
4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.56
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.62
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
1.07
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
4.55
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00015
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.015
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.067
-
alpha-1,5-L-arabinofuranobiose mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.09
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.18
-
alpha-1,5-L-arabinofuranobiose mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.22
-
4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
0.33
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
1.78
-
alpha-1,5-L-arabinofuranobiose mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
4.14
-
4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
11.15
-
alpha-1,5-L-arabinofuranobiose mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
63.6
-
alpha-1,5-L-arabinofuranobiose wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis