Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21gold (DE3) cells | Streptomyces avermitilis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.8 M sodium citrate, 0.2 M sodium chloride, and 0.1 M Tris, pH 7.0 | Streptomyces avermitilis |
Protein Variants | Comment | Organism |
---|---|---|
D135A | inactive | Streptomyces avermitilis |
D135N | inactive | Streptomyces avermitilis |
D20A | inactive | Streptomyces avermitilis |
D20N | the mutant shows 0.7% of wild type activity | Streptomyces avermitilis |
E196A | inactive | Streptomyces avermitilis |
L289A | the mutant shows 10% of wild type activity | Streptomyces avermitilis |
N159A | the mutant shows 1.5fold increased activity compared to the wild type | Streptomyces avermitilis |
N159L | the mutant shows 19fold increased activity compared to the wild type | Streptomyces avermitilis |
Y192A | the mutant shows 40% of wild type activity | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.5 | - |
4-nitrophenyl alpha-L-arabinofuranoside | wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
1.1 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
1.7 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
11.5 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
40.7 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces avermitilis | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
alpha-1,5-L-arabinofuranobiose + H2O | - |
Streptomyces avermitilis | L-arabinofuranose | - |
? | |
additional information | the enzyme shows no activity towards alpha-1,2-L-arabinofuranobiose and alpha-1,3-L-arabinofuranobiose | Streptomyces avermitilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Streptomyces avermitilis |
Araf43A | - |
Streptomyces avermitilis |
exo-1,5-alpha-L-arabinofuranosidase | - |
Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.11 | - |
4-nitrophenyl alpha-L-arabinofuranoside | wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.56 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.62 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
1.07 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
4.55 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.015 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.067 | - |
alpha-1,5-L-arabinofuranobiose | mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.09 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.18 | - |
alpha-1,5-L-arabinofuranobiose | mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.22 | - |
4-nitrophenyl alpha-L-arabinofuranoside | wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
0.33 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
1.78 | - |
alpha-1,5-L-arabinofuranobiose | mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
4.14 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
11.15 | - |
alpha-1,5-L-arabinofuranobiose | mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis | |
63.6 | - |
alpha-1,5-L-arabinofuranobiose | wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C | Streptomyces avermitilis |