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Literature summary for 3.2.1.55 extracted from

  • Lee, J.H.; Hyun, Y.J.; Kim, D.H.
    Cloning and characterization of alpha-L-arabinofuranosidase and bifunctional alpha-L-arabinopyranosidase/beta-D-galactopyranosidase from Bifidobacterium longum H-1 (2011), J. Appl. Microbiol., 111, 1097-1107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bifidobacterium longum

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 68% residual activity at 1 mM Bifidobacterium longum
Co2+ 54.4% residual activity at 1 mM Bifidobacterium longum
Cu2+ 7% residual activity at 1 mM Bifidobacterium longum
Fe2+ 81.3% residual activity at 1 mM Bifidobacterium longum
Mg2+ 80.2% residual activity at 1 mM Bifidobacterium longum
Ni2+ 85.1% residual activity at 1 mM Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium acetate buffer (pH 5.5), at 37°C Bifidobacterium longum
1.73
-
ginsenoside Rc in 50 mM sodium acetate buffer (pH 5.5), at 37°C Bifidobacterium longum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 109.4% relative activity at 1 mM Bifidobacterium longum
Na+ the enzyme activity is unaffected by NaCl concentration in the range of 0-1 mM Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum E7CY70
-
-
Bifidobacterium longum H-1 E7CY70
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-charged HiTrap IMAC column chromatography Bifidobacterium longum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.64
-
crude extract, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C Bifidobacterium longum
71.36
-
after 26fold purification, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O 100% activity Bifidobacterium longum 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O 100% activity Bifidobacterium longum H-1 4-nitrophenol + alpha-L-arabinofuranose
-
?
ginsenoside Rc + H2O 10.5% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside Bifidobacterium longum ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O 10.5% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside Bifidobacterium longum H-1 ginsenoside Rd + L-arabinofuranose
-
?
additional information the enzyme does not hydrolyse 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinofuranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and ginsenoside Rb2 Bifidobacterium longum ?
-
?
additional information the enzyme does not hydrolyse 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinofuranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and ginsenoside Rb2 Bifidobacterium longum H-1 ?
-
?

Synonyms

Synonyms Comment Organism
AfuB
-
Bifidobacterium longum
alpha-L-arabinofuranosidase
-
Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
when incubated for 15 min at pH 7.0 Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
at 37°C Bifidobacterium longum