Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bifidobacterium longum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 68% residual activity at 1 mM | Bifidobacterium longum | |
Co2+ | 54.4% residual activity at 1 mM | Bifidobacterium longum | |
Cu2+ | 7% residual activity at 1 mM | Bifidobacterium longum | |
Fe2+ | 81.3% residual activity at 1 mM | Bifidobacterium longum | |
Mg2+ | 80.2% residual activity at 1 mM | Bifidobacterium longum | |
Ni2+ | 85.1% residual activity at 1 mM | Bifidobacterium longum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
4-nitrophenyl alpha-L-arabinofuranoside | in 50 mM sodium acetate buffer (pH 5.5), at 37°C | Bifidobacterium longum | |
1.73 | - |
ginsenoside Rc | in 50 mM sodium acetate buffer (pH 5.5), at 37°C | Bifidobacterium longum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 109.4% relative activity at 1 mM | Bifidobacterium longum | |
Na+ | the enzyme activity is unaffected by NaCl concentration in the range of 0-1 mM | Bifidobacterium longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum | E7CY70 | - |
- |
Bifidobacterium longum H-1 | E7CY70 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-charged HiTrap IMAC column chromatography | Bifidobacterium longum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.64 | - |
crude extract, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C | Bifidobacterium longum |
71.36 | - |
after 26fold purification, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C | Bifidobacterium longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | 100% activity | Bifidobacterium longum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | 100% activity | Bifidobacterium longum H-1 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | 10.5% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside | Bifidobacterium longum | ginsenoside Rd + L-arabinofuranose | - |
? | |
ginsenoside Rc + H2O | 10.5% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside | Bifidobacterium longum H-1 | ginsenoside Rd + L-arabinofuranose | - |
? | |
additional information | the enzyme does not hydrolyse 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinofuranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and ginsenoside Rb2 | Bifidobacterium longum | ? | - |
? | |
additional information | the enzyme does not hydrolyse 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinofuranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and ginsenoside Rb2 | Bifidobacterium longum H-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AfuB | - |
Bifidobacterium longum |
alpha-L-arabinofuranosidase | - |
Bifidobacterium longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
when incubated for 15 min at pH 7.0 | Bifidobacterium longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
at 37°C | Bifidobacterium longum |