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Literature summary for 3.2.1.55 extracted from
- Functional and biophysical characterization of a hyperthermostable GH51 alpha-L-arabinofuranosidase from Thermotoga petrophila (2011), Biotechnol. Lett., 33, 131-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)DELTASlyD cells | Thermotoga petrophila |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 100 mM bis-Tris pH 5.5, 200 mM ammonium acetate, 40% (v/v) 2-methyl-2,4 pentanediol and 1% (v/v) dioxane | Thermotoga petrophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000 | Thermotoga petrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga petrophila | A5IKD0 | - |
- |
| Thermotoga petrophila RKU-1 | A5IKD0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-charged HiTrap Chelating HP column chromatography and Superdex 200 gel filtration | Thermotoga petrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-1,5-linked arabinoheptaose + H2O | - |
Thermotoga petrophila | L-arabinose + ? | - |
? |  |
| alpha-1,5-linked arabinoheptaose + H2O | - |
Thermotoga petrophila RKU-1 | L-arabinose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000 | Thermotoga petrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Thermotoga petrophila |
| AraF | - |
Thermotoga petrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermotoga petrophila |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 90 | - |
Thermotoga petrophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
the enzyme shows no loss of activity after 20 h at 90°C | Thermotoga petrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermotoga petrophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Thermotoga petrophila |
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