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Literature summary for 3.2.1.55 extracted from

  • Lagaert, S.; Pollet, A.; Delcour, J.A.; Lavigne, R.; Courtin, C.M.; Volckaert, G.
    Substrate specificity of three recombinant alpha-L-arabinofuranosidases from Bifidobacterium adolescentis and their divergent action on arabinoxylan and arabinoxylan oligosaccharides (2010), Biochem. Biophys. Res. Commun., 402, 644-650.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bifidobacterium adolescentis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, recombinant isoform AXH-d3, SDS-PAGE Bifidobacterium adolescentis
63000
-
x * 63000, recombinant isoform AbfA, SDS-PAGE Bifidobacterium adolescentis
87000
-
x * 87000, recombinant isoform AbfB, SDS-PAGE Bifidobacterium adolescentis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arabinoxylan oligosaccharides + H2O Bifidobacterium adolescentis
-
L-arabinose + ?
-
?
barley arabinoxylan + H2O Bifidobacterium adolescentis on barley arabinoxylan-0.76 the combination of isoform AbfA and AXH-d3 cleaves 35% of the total arabinose L-arabinose + ?
-
?
rye arabinoxylan + H2O Bifidobacterium adolescentis on rye arabinoxylan-0.52 the combination of isoform AbfA and AXH-d3 cleaves 30% of the total arabinose L-arabinose
-
?
sugar beet arabinan + H2O Bifidobacterium adolescentis isoforms AbfB and AXH-d3 cleave small amounts of L-arabinose from sugar beet arabinan (4% and 2% of the total arabinoses, respectively) L-arabinose + ?
-
?
wheat arabinoxylan + H2O Bifidobacterium adolescentis recombinant isoform AbfA acts synergistically with isoforms AbfB and AXH-d3. In combination with AXH-d3, it releases 60% of L-arabinose from wheat arabinoxylan. Isoform AbfA does not act on di-linked arabinoses, but does release mono(2)-linked arabinoses present in wheat arabinoxylan-0.51 L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Bifidobacterium adolescentis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Bifidobacterium adolescentis 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O substrate for isoform AbfA Bifidobacterium adolescentis 4-nitrophenol + beta-D-xylopyranose
-
?
arabinoxylan oligosaccharides + H2O
-
Bifidobacterium adolescentis L-arabinose + ?
-
?
barley arabinoxylan + H2O on barley arabinoxylan-0.76 the combination of isoform AbfA and AXH-d3 cleaves 35% of the total arabinose Bifidobacterium adolescentis L-arabinose + ?
-
?
additional information isoform AbfB shows no activity with 4-nitrophenyl beta-D-xylopyranoside, isoforms AbfB and AXH-d3 are not active on debranched arabinan. All three isozymes, AfbA, AfbB and AXH-d3, lack activity against xyloglucan Bifidobacterium adolescentis ?
-
?
rye arabinoxylan + H2O on rye arabinoxylan-0.52 the combination of isoform AbfA and AXH-d3 cleaves 30% of the total arabinose Bifidobacterium adolescentis L-arabinose
-
?
sugar beet arabinan + H2O isoforms AbfB and AXH-d3 cleave small amounts of L-arabinose from sugar beet arabinan (4% and 2% of the total arabinoses, respectively) Bifidobacterium adolescentis L-arabinose + ?
-
?
wheat arabinoxylan + H2O recombinant isoform AbfA acts synergistically with isoforms AbfB and AXH-d3. In combination with AXH-d3, it releases 60% of L-arabinose from wheat arabinoxylan. Isoform AbfA does not act on di-linked arabinoses, but does release mono(2)-linked arabinoses present in wheat arabinoxylan-0.51 Bifidobacterium adolescentis L-arabinose + ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, recombinant isoform AXH-d3, SDS-PAGE Bifidobacterium adolescentis
? x * 63000, recombinant isoform AbfA, SDS-PAGE Bifidobacterium adolescentis
? x * 87000, recombinant isoform AbfB, SDS-PAGE Bifidobacterium adolescentis

Synonyms

Synonyms Comment Organism
AbfA recombinant isoform Bifidobacterium adolescentis
AbfB recombinant isoform Bifidobacterium adolescentis
alpha-L-arabinofuranosidase
-
Bifidobacterium adolescentis
AXH-d3 recombinant isoform Bifidobacterium adolescentis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
optimal temperature for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside is 50 °C for recombinant isoform AbfB Bifidobacterium adolescentis
50
-
optimal temperature for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside is 50 °C for recombinant isoform AbfA Bifidobacterium adolescentis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
optimal pH value for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside for recombinant isoforms AbfA and AbfB Bifidobacterium adolescentis