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Literature summary for 3.2.1.55 extracted from

  • Chlubnova, I.; Filipp, D.; Spiwok, V.; Dvorakova, H.; Daniellou, R.; Nugier-Chauvin, C.; Kralova, B.; Ferrieres, V.
    Enzymatic synthesis of oligo-D-galactofuranosides and L-arabinofuranosides: from molecular dynamics to immunological assays (2010), Org. Biomol. Chem., 8, 2092-2102.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme might be useful for enzymatic synthesis of galactosylfuranoside-containing pharmacophores to be used in mammals Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-L-arabinofuranosyl-1,4-alpha-D-xylopyranoside + H2O Acetivibrio thermocellus
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L-arabinofuranose + D-xylopyranose
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + L-arabinofuranose also synthesis of regioisomers of di- to pentaarabinofuranosides ?
alpha-L-arabinofuranosyl-1,4-alpha-D-xylopyranoside + H2O
-
Acetivibrio thermocellus L-arabinofuranose + D-xylopyranose
-
?
additional information the enzyme recognizes both the L-Araf motif and its D-Galf analogue. The arabinofuranosidase is also able to hydrolyze galactosyl derivatives and is efficient in catalyzing oligomerisations using 4-nitrophenyl furanosides as donors. Molecular dynamics and active site structure determination and analysis by NMR, substrate specificity, and product structures by mass spectrometry and NMR.overview Acetivibrio thermocellus ?
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?

Synonyms

Synonyms Comment Organism
arabinofuranosyl hydrolase
-
Acetivibrio thermocellus
Araf 51
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
82
-
-
Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Acetivibrio thermocellus